Optical Projection Tomography - OPT

Optical projection tomography (OPT) core facility is a part of the Biocenter Finland activities and is integrated to Oulu BioImaging (OBI) and Tissue Imaging Center (TIC) and the Transgenic animals core facility.

OPT is a 3D imaging technology for biological specimens up to 1x1,5cm in size.

It is a convenient method for viewing the 3D anatomy, gene and protein expression patterns during embryo development, or separate organs and biopsies.

We are using Bioptonics 3001 device with the resolution 1024x1024 (~3,1micron) which allows analyzing specimens in 4 channels:

  1. Bright field: in situ hybridization specimens (BMP purple, NBT/BCIP), beta-galactosidase and other stains;
  2. GFP1 425/40nm exciter, 475nm LP emitter: auto-fluorescence and general morphology;
  3. GFP 470/40nm exciter, 515nm LP emitter;
  4. Texas red 560/40nm exciter, 610nm LP emitter.

The raw data obtained from scanner is further processed and analyzed by the 3D analysis software. TIC has license for the Bitplane Imaris 7.4 scientific software that allows data visualization and detailed morphometric studies of the OPT specimen.

We are offering:

Tissue staining for the OPT (immuno-, beta-gal ect.)
Sample preparation for OPT: embedding and clearing
Scanning and reconstruction
Data analysis (Quantification, 3D shape measurements, 3D and 2D linear measurements)
Recovery of the specimens for the following use (histology analysis, IHC & ect.)

Please contact us to discuss about your specimen preparation and requests.

Fees at the OPT core facility:

Fee information.

Reservation of the Bioptonics 3001 device:

Asimov reservation page.

Responsible scientists:

Veli-Pekka Ronkainen, Ph.D.
tel. +358-(0)294 486114
Room: 408B
E-mail: veli-pekka.ronkainen (at) oulu.fi

Technicians:

Hannele Härkman
Tel.: +358-(0)294 486089
E-mail: Hannele.Härkman (at) oulu.fi

Johanna Kekolahti-Liias
Tel.: +358-(0)294 486089
E-mail: Johanna.Kekolahti-Liias (at) oulu.fi

Street address:

Biocenter Oulu, room 414A
Aapistie 5A,
FIN-90220 Oulu,
Finland

Last updated: 27/6/2016