Details and specifications for Agilent 2100 bioanalyzer assays

Samples have to be delivered in a suitable buffer and concentration. 

Following kits are available:

Agilent High Sensitivity DNA Kit

Separation, sizing and quantification of low concentrated dsDNA samples from 50 to 7000 bp.

Analytical specifications

High Sensitivity DNA Kit

Sizing range

50 – 7000 bp

Sizing resolution

50-600 bp: ±10%
600-7000 bp:± 20%

Sizing accuracy

± 10% 

Sizing reproducibility

5% CV

Quantitation accuracy

20%

Quantitation reproducibility

50-2000 bp: 15% CV
2000-7000 bp: 10% CV

Quantitative range

5-500 pg/µL

Maximum salt concentration in sample

10 mM Tris and 1 mM EDTA

Samples per chip

11

%CV= coefficient of variance

Agilent DNA 1000 Kit

For the separation, sizing and quantification of dsDNA fragments from 25 to 1000 bp.

Analytical specifications

DNA 1000 Kit

Sizing range

25–1000 bp

Sizing resolution

25–100 bp: 5 bp
100–500 bp: 5%
500–1000 bp: 10%

Sizing accuracy

± 10%

Sizing reproducibility

5% CV

Quantitation accuracy

20%

Quantitation reproducibility

25–500 bp:15% CV
500-1000 bp:5% CV

Quantitative range

0.5-50 ng/µL

Maximum salt concentration in sample

250 mM for KCl,
15 mM for MgCl2,
250 mM NaCl

Samples per chip

12

%CV= coefficient of variance

Agilent RNA 6000 Nano Kit

For the analysis and quantitation of total and mRNA samples of 25 to 500 ng/µL in concentration.

Analytical specifications

RNA 6000 Nano Kit

total RNA

mRNA

Quantitative range

25 - 500 ng/µL

25 - 250 ng/µL

Qualititative range

5 - 500 ng/µL

5 - 250 ng/µL

Sensitivity (S/N>3)

5 ng/µL in water

25 ng/µL in water

Quantitation reproducibility
(within a chip)

10% CV

10% CV

Quantitation accuracy

20%

20%

Maximum sample buffer strength

100 mM Tris
0.1 mM EDTA
or 125 mM NaCl
15 mM MgCl2

100 mM Tris
0.1 mM EDTA
or 125 mM NaCl
15 mM MgCl2

Samples per chip

11

11

%CV= coefficient of variance

Last updated: 27.3.2017