DEPARTMENT OF MEDICAL BIOCHEMISTRY AND MOLECULAR BIOLOGY
Professor Johanna Myllyharju, Ph.D.
Selected publications
- Myllyharju, J.& Kivirikko, K.I.: Characterization of the iron- and 2-oxoglutarate-binding sites of human prolyl 4-hydroxylase. EMBO J. 16: 1173-1180, 1997.
- Vuorela, A., Myllyharju, J., Nissi, R., Pihlajaniemi, T. & Kivirikko, K.I.: Assembly of human prolyl 4-hydroxylase and type III collagen in the yeast Pichia pastoris: formation of a stable enzyme tetramer requires coexpression with collagen and assembly of a stable collagen requires coexpression with prolyl 4-hydroxylase. EMBO J. 16: 6702-6712, 1997.
- Myllyharju, J. & Kivirikko, K.I.: Identification of a novel proline-rich peptide-binding domain in prolyl 4-hydroxylase. EMBO J. 18: 306-312, 1999.
- Hirsilä, M., Koivunen, P., Günzler, V., Kivirikko, K.I. & Myllyharju, J.: Characterization of the human prolyl 4-hydroxylases that modify the hypoxia-induciple factor. J. Biol. Chem. 278: 30772-30780, 2003.
- Myllyharju, J. & Kivirikko, K.I.: Collagens, modifying enzymes and their mutations in humans, flies and worms. Trends Genet. 20: 33-43, 2004.
- Rautavuoma, K., Takaluoma, K., Sormunen, R., Myllyharju, J., Kivirikko, K.I. & Soininen, R.: Premature aggregation of type IV collagen and early lethality in lysyl hydroxylase 3 null mice. Proc. Natl. Acad. Sci. USA 101: 14120-14125, 2004.
- Pekkala, M., Hieta, R., Bergmann, U., Kivirikko, K.I., Wierenga, R.K. & Myllyharju, J.: The peptide-substrate-binding domain of collagen prolyl 4-hydroxylases is a tetratricopeptide repeat domain with functional aromatic residues. J. Biol. Chem. 279: 52255-52261, 2004.
- Hirsilä, M., Koivunen, P., Xu, L., Seeley, T., Kivirikko, K.I. & Myllyharju, J.: Effect of desferrioxamine and metals on the hydroxylases in the oxygen sensing pathway. FASEB J. 19: 1308-1310, 2005.
- Keskiaho, K., Hieta, R., Sormunen, R. & Myllyharju, J.: Clamydomonas reinhardtii has multiple prolyl 4-hydroxylases, one of which is essential for proper cell wall assembly. Plant Cell 19, 256-269, 2007.
- Holster, T., Pakkanen, O., Soininen, R., Sormunen, R., Nokelainen, M., Kivirikko, K.I. & Myllyharju, J.: Loss of assembly of the main basement membrane collagen, type IV, but not fibril-forming collagens and embryonic death in collagen prolyl 4-hydroxylase I null mice. J. Biol. Chem. 282: 2512-2519, 2007.