ITC is a thermodynamic technique that directly measures heat released or absorbed during biochemical binding events. It determines the relative binding affinity (KD), reaction stoichiometry (n) and enthalpy change (ΔH). In addition entropy change (ΔS) and Gibbs free energy change (ΔG) and change in heat capacity of binding (ΔCp) can be determined. The data provides deeper insights into structure-function relationships and binding mechanisms
More detailed information can be found from the manufacturer web page.
- The sample and titrant must be in exactly the same buffer. Changing the sample buffer by dialysis or other suitable methods if there are differences in the buffer composition.
- Titrant concentration is usually 10x higher than sample concentration.
- Buffers with high ionization energy are not recommended. To obtain good results sample titration vs. buffer should be run as reference if there are any effects coming from the buffer.
- Required volume: ITC-200: titrant 50µl, sample 300µl
Customers inside FBMM are suggested to use NOTIO for the reservation. Customers from outside can fill the submission form and send it to the contact person before the analysis.
For national academic user: 110 €/use/day (excl. taxes).
For other users: 220 €/use/day (excl. taxes).
For frequent users: fixed annual fee (to be negotiated with the Core Facility).
Applications of long-term collaboration and special prices should be submitted directly to the chief of the Core Facility, Prof. Lloyd Ruddock.
Acknowledging the BCO core facility
The Oulu BCO core facilities are supported by funding from Biocenter Finland, Biocenter Oulu, the University of Oulu, FBMM, and regional funding. We kindly ask the users to acknowledge this resource whenever publications are being finalised, for example:
"The use of the facilities and expertise of the Biocenter Oulu biophysical protein analysis core facility, a member of Biocenter Finland, is gratefully acknowledged."
In addition we ask the users to inform us whenever such publications have been accepted for publication.
Dr. Hongmin Tu
Faculty of Biochemistry and Molecular Medicine
University of Oulu
Aapitie 7B, 90014 Oulu, Finland
tel. +358-(0)294 485821
e-mail: hongmin.tu (at) oulu.fi
(Pictures obtained from GE Healthcare, Bionavis, Applied Photophysics and own research, obtained under permission of owners)
Last updated: 6.2.2018