Protein purification

FBMM has a highly developed infrastructure for the purification of recombinant proteins. The facility hosts several purification systems (Table 1) as well as  a dedicated lab for protein purification (F205), which also includes a Nanodrop 1000 spectrophotometer, gel documentation device ChemiDoc^TM XRS⁺( Bio-Rad) and other devices related to protein purification. Many devices, especially those locating in the protein purification lab, are also available to non-academic users (Table 1). For the academic users we can provide help for: i) planning your protein purification protocol, ii) running your experiments, and iii) analyzing your results (for a basic price or via collaboration). For regular or large-scale purifications research groups must provide required consumables by themselves. Some consumables, like columns, certain column material and SDS PAGE system, we can only provide for test runs. For the commercial users the devices will be provided using an hour- or daily-based rent depending on the device. Weekly or monthly “package offers” for a set of specific devices including working place in the F205 laboratory are also provided if needed.

Table 1. Protein purification units in FBMM.  All systems except Bio-Rad Biologic LP are operated via a computer, and include monitors detecting UV and conductivity values. All systems are also connected to fraction collectors. The devices marked by a star are also available to outside users.

instrument	temperature (range)	laboratory	special detectors	person-in-charge of the device
*Äkta Explorer	RT	F205		Hongmin Tu
*Äkta Purifier	10°C-RT	F225	SLS RI	Ville Ratas Rajaram Venkatesan
*Äkta Purifier	RT	F205		Kristian Koski
*Shimadzu HPLC purification package	4°C-10°C	F205	SLS fluorescence RI	Rahul Nenakar Kristian Koski Ville Ratas
*Bio-Rad Biologic DuoFlow	4°C-10°C	F225		Shiv Kumar Sah-Teli
Bio-Rad Biologic DuoFlow	4°C-10°C	F224		Matti Myllykoski
Bio-Rad Biologic DuoFlow	4°C-10°C	F124		Teemu Haikarainen
Bio-Rad Biologic DuoFlow	RT	F124		Teemu Haikarainen
Bio-Rad Biologic DuoFlow	4°C-10°C	L041		Henni Piirainen
*Bio-Rad, Biologic, LP	4°C-10°C	F205	only UV	Rajaram Venkatesan

 

Shimadzu-HPLC in F205. Operated in a cold cabin.

 

Äkta Purifier in F205. Operated at room temperature.

 

The starting point for these projects will be a planning meeting with Kristian Koski and Ville Ratas.

Contact information:

Kristian Koski / Ville Ratas
Faculty of Biochemistry and Molecular Medicine
Aapistie 7A
Fi-90220 Oulu
Office: F216A
Tel: +358-294481182
E-mail: kristian.koski (at) oulu.fi / ville.ratas (at) oulu.fi