Services

Crystallization test

  • The crystallization of one protein is tested in two temperatures. The service setup includes two well-plates with 96 crystallization conditions / plate
  • The service is a pretest for a broader service (Screening of crystallization conditions or Optimization of crystallization condition)
  • The successful execution of the service requires purified protein solution (1-10 mg/ml), which is needed at least 5,5 µl / well-plate (optimum 40 µl / well-plate)
  • The customer receives preliminary information about the crystallizability of the protein (and possibly suitable crystals for the collection of diffraction data)
  • The service price is 115 € / protein
  • The service can be initiated within one week after protein delivery and completed within one week after initiation
  • Additional services:
      • The quality check of protein sample with SLS instrument 110 €
      • The optimization of protein sample buffer content with DLS instrument 60 €
      • Study of protein stability with DSF instrument 70 €

Screening of crystallization conditions

  • The crystallization of one protein is screened in different conditions. The service setup includes at maximum 576 crystallization conditions / temperature
  • The temperatures used are +4 °C and +22 °C
  • The progression of the crystallization in every condition is photographed once a day for the first week and once a week after that.
  • The customer receives information about the crystallizability of the protein (and possibly suitable crystals for the collection of diffraction data). The customer can follow the progression of the crystallizations through IceBear software.
  • The service price is 45 € / well-plate (minimum 4 well-plates)
  • The service can be initiated within one week after protein delivery and completed within one month after the initiation
  • Additional services:
      • Optimization of crystallization condition
      • Special conditions according to separate offer

Optimization of crystallization condition

  • The crystallization of one protein is optimized, or the quality and size of protein crystals identified  in earlier conditions are being improved
  • The service is a continuation for Crystallization test or Screening of crystallization conditions
  • The successful execution of the service requires purified protein solution (1-10 mg/ml), which is needed at least 5,5 µl / well-plate (optimum 40 µl / well-plate)
  • The customer receives suitable crystals for the collection of diffraction data or conditions for additional optimization of crystallization
  • The service price is 120 € / protein
  • The service can be initiated within one week after protein delivery and completed within one month after the initiation
  • Additional services:
      • Collection of diffraction data
      • Special conditions according to separate offer (e.g. additives, seeding)

Characterization of a crystal

  • One crystal is characterized under the x-ray beam
  • The crystal emerged in Crystallization test, Screening of crystallization conditions or Optimization of crystallization condition is picked from well-plate and the diffraction is tested wig X-ray beam
  • The service reveals the quality of the crystal, resolution and possibly the symmetry and size of the unit cell
  • The customer receives information about the suitability of the crystal to the structural determination of the protein (if the crystal was protein)
  • The service price is 90 €
  • The service will be delivered within a week
  • Additional services:
      • Collection and processing of crystal data
      • Characterization of an additional crystal (during the same day)  30 € / crystal

Collection and processing of crystal data

  • The diffraction data is collected from one protein crystal and the data is processed for structure determination
  • The service requires Characterization of a crystal
  • The protein crystal is centered to the x-ray beam for test measurements (2 angles, 5 pictures), and the strategy for overnight data collection is determined
  • The customer receives a report and processed crystal data for protein structure determination
  • The service price is 150 €
  • The service will be delivered within a week
  • Additional services:
      • Additional data collection from the same crystal 120 € / 24 h
      • Primary survey of the crystal data 70 €

Determination of protein structure

  • Protein structure is determined from protein crystal data
  • Crystal data can be collected by X-ray diffractometry or synchrotron
  • The service includes a kick-off meeting with the customer to agree the content and milestones of the service
  • The customer receives the coordinates of the solved protein structure
  • The service price depends on the case and is base on the rate 70 € / h
  • The service delivery time depends on the case

Last updated: 20.12.2018