Laboratories, equipment and their booking

Booking laboratory room and equipment

Researchers may reserve a laboratory space, an isoflurane anaesthesia device, IVC-cages or an isolator.

  • The laboratory space is booked through the booking list on the door of the room or in the booking log
  • The isoflurane anaesthesia device will be booked in the booking log in the laboratory
  • Need for using the IVC-cages should be negotiated with the research technician (Tuula.Stranius(at)oulu.fi)
  • Need for using the isolator should be negotiated with the research technician (Tuula.Stranius(at)oulu.fi)
  • Using the IVIS devices must be agreed upon with Veli-Pekka Ronkainen (Veli-Pekka.Ronkainen(at)oulu.fi)
  • Using the MicroCT devices must be agreed upon with Mikko Finnilä (mikko.finnila(at)oulu.fi) live animals and ja Sakari Karhula (sakari.karhula(at)oulu.fi) other material

Quarantine unit

The quarantine unit is a closed area with restricted personnel traffic. In this department, such mice and rats that cannot be brought into the research unit due to their microbiological quality, are maintained in IVCs or isolators. The unit has two animal rooms and a laboratory room with a laminar flow cabinet.

Strains of mice waiting for rederivation by embryo transfer are kept in the quarantine unit, and it is also possible to perform short-term animal experiments there.

The need for maintaining animals in the quarantine unit is decided with the veterinarian and the research technician when planning animal import.

Individually ventilated cages

Individually ventilated cages is a system, where every cage has own ventilation and the cage lid is covered with the hepa-filter. The air pressure in IVCs is kept positive for clean rodents, and negative for contaminated animals. The cage change is performed under the laminar change station and the animals are not in contact with the ordinary room air. It is possible to keep animals with different microbiological quality, within the same IVC system.

There are some IVCs in the research unit, for the use of researchers. IVC offers a possibility to use the animals under experiments in the conventional unit, in the similar microbiological level as in the barrier. It is advisable to keep the immunodeficient animals in IVCs.

The need for maintaining animals in the quarantine unit is decided with the veterinarian and the research technician when planning animal import.

Isolators

An isolator is a space confined from an external space where both clean rodents (positive pressure isolator) and contaminated rodents (negative pressure isolator) can be maintained. The supply and exhaust air is hepa-filtered and traffic of goods is conducted through a sealable gate. There is no direct contact with the animals.

KEKS has several isolators at its disposal. For example, immune-deficient mice or mice waiting for embryo transfer are maintained in the isolators.

The need for using the isolator is decided with the veterinarian and the research technician when planning animal import and experiments.

Imaging

Several different imaging devices have been installed in the KEKS research unit for the purpose of imaging the laboratory animals. The IVIS and the Micro-CT can be used by the researchers. Reetta Vuolteenaho (Reetta Vuolteenaho(at)oulu.fi) is responsible for the use and user training for the IVIS device.

Laboratories and procedure rooms

It is possible to perform procedures on small rodents and process the samples in the large laboratory space in KEKS. There is a laminar flow cabinet in the space.

Experimental procedures on small rodents can be performed in the procedure rooms. There are two isoflurane anaesthesia devices for small rodents.

Last updated: 30.12.2016