Surface Plasmon Resonance

Introduction

Surface Plasmon Resonance (SPR) is a label-free technology for biomolecular interaction analysis. It monitors the formation and dissociation of biomolecular complexes on a sensor surface, where the ligand molecule is immobilized. The real-time binding measurement is performed by injecting the testing analyte in solution. This technology is widely used in ligand study, drug discovery and development. The facility is equipped with two different SPR instruments:

SPR - Biacore T200 (Biacore AB, Ge Healthcare)

Biacore T200 has measurement capacities ranging from 103-109 M-1s-1 for the association constant and 10-1-10-6 s-1 for the dissociation constant. Minimum sample concentration is 10pM. This instrument also  has a possibility to measure thermodynamic parameters.

MP-SPR – Navi-220A (Bionavis)

Navi-220A is a multiparametric SPR instrument that has extended capacities compared to the conventional SPR instruments. It is not limited only to the water based media in flow cell and bio-application. The application can expand to analyzinge substances in any media , e.g. water,gases and air. The chip surface could be modified  in many different ways. Instead of the thin layer of gold in Biacore series Bionavis chips can be coated with any metals, organic or inorganic materials. This instrument ia able to provide more physical information about the surfaces as refractive indexes and layer thickness, etc. The measuring capacities range from 103-108 M-1s-1 for the association constant and 100-10-5 s-1 for the dissociation constant.

Sample requirements

All the SPR instruments are equipped with auto-sampler and racks for up to 96 samples. A standard analysis is composed of 4 steps:

  1. Sample preparation – purification, dilutions etc.
  2. Chip preparation – one of interactants must be immobilized on the chip surface using amine-, thiol-, aldehyde- coupling, or Tag-binding, e.g. His, Biotin, GST, etc.   The ligand (immobilized on the chip) should have 100-200µl with a concentration of 1-100µg/ml. The immobilization buffer  should not contain any “reactive groups”,
  3. Injections – several injections of the analytes with different concentrations ranging from 0.1x to 10x of the expected binding affinity. Insert a chip regeneration step between each analyte injection. The running buffer for the interaction analysis can be phosphate, HEPES, or TRIS buffers. Minimum 100ml is needed for one experiment.
  4. Data analysis – fitting with predefined model.

Click here for Biacore T200 system details.

Sample submission

Customers inside FBMM are suggested to use NOTIO for the reservation. Customers from outside can fill the submission form and send it to the contact person before the analysis.

Click here for Sample Submission Form.

Prices

For national academic users: 110 €/use/day (excl. taxes).

For other users: 220 €/use/day (excl. taxes).

Uses of chips are not included in the price. The cost will be calculated separately according to the chip purchasing prices.

Applications of long-term collaboration and special prices should be submitted directly to the chief of the Core Facility, Prof. Lloyd Ruddock.

Acknowledging the BCO core facility

The Oulu BCO core facilities are supported by funding from Biocenter Finland, Biocenter Oulu, the University of Oulu, FBMM, and regional funding.  We kindly ask the users to acknowledge this resource whenever publications are being finalised, for example:

"The use of the facilities  and expertise of the Biocenter Oulu core facility, a member of Biocenter Finland, is gratefully acknowledged."

In addition we ask the users to inform us (i) whenever such publications have been accepted for publication.

Contact person:

Dr. Hongmin Tu
Faculty of Biochemistry and Molecular Medicine
University of Oulu
Aapitie 7B, 90014 Oulu, Finland
tel. +358-(0)294 485821
e-mail: hongmin.tu(at)oulu.fi

Last updated: 18.8.2017