Mechanisms and applications of disulfide bond formation
Prof. Lloyd Ruddock, Ph.D.
Biocenter Oulu and Faculty of Biochemistry and Molecular Medicine
P.O.Box 3000, FIN-90014 University of Oulu, Finland
University of Oulu, Aapistie 7A, 90220 Oulu, Finland
Tel: +358-(0)294 481683,
e-mail: lloyd.ruddock (at) oulu.fi
About the project
The research undertaken in this project aims to develop efficient systems for the production of disulfide bond containing proteins in the cytoplasm of E.coli and combine these with systems under development for N-glycosylation and secretion of folded proteins to form second generation protein cell factories. Gen2Co would have the benefits of E.coli production systems plus those of eukaryotic expression systems in terms of PTM and allows the additional advantage of homogenous N-glycosylation status. Our system is already approaching levels of CHO for the production of aglycosylated full length antibodies.
Last updated: 11/4/2017