Rikkert K. Wierenga, Ph.D.

Structural Enzymology: A Quantitative Approach

Project leader
Prof. Rikkert K. Wierenga, Ph.D.

Affiliation
Biocenter Oulu and Faculty of Biochemistry and Molecular Medicine, University of Oulu

Mailing address
P.O.Box 3000, FIN-90014 University of Oulu, Finland

Street address
University of Oulu, Aapistie 7A, 90220 Oulu, Finland

Tel. +358-(0)294 481199,
e-mail: rik.wierenga (at) oulu.fi

About the project

The ongoing structural enzymological studies of collagen prolyl 4-hydroxylase (C-P4H, α2β2, 240kD) and trifunctional enzyme (TFE, α2β2, 240kD) remain challenging. The TFEs are acyl-CoA dependent enzymes, catalysing three steps of the β-oxidation pathway. Other CoA, thioester dependent enzymes that are being studied concern multifunctional enzyme, type-1 (MFE1) and the monofunctional enzymes enoyl-CoA-hydratase, enoyl-CoA-isomerase and thiolase. In each of these projects we study all aspects of the reaction mechanisms ranging from processivity (C-P4H), substrate channeling (TFE, MFE1) to the role of hydrogen bond networks in the active sites, using a multidisciplinary approach including biocomputing, chemistry and structural enzymology.

Project details

  1. Background and Significance
  2. Recent Progress
  3. Future Goals
  4. Publications 2016-
  5. Research Group Members
  6. National and International Activities

Last updated: 25.4.2017