Structural Enzymology: A Quantitative Approach

Project leader
Prof. Rikkert K. Wierenga, Ph.D.

Affiliation
Biocenter Oulu and Faculty of Biochemistry and Molecular Medicine, University of Oulu

Mailing address
P.O.Box 3000, FIN-90014 University of Oulu, Finland

Street address
University of Oulu, Aapistie 7A, 90220 Oulu, Finland

Tel. +358-(0)294 481199,
e-mail: rik.wierenga (at) oulu.fi

Focus area spearhead project web page
http://www.oulu.fi/university/node/49041

About the project

Our studies concern the characterisation of enzymes at the molecular level, including enzyme-substrate interactions. We study properties of CoA-dependent, thioester dependent lipid metabolising enzymes, such as acyl-CoA dehydrogenases, enoyl-CoA isomerases (ECIs), thiolases, multifunctional enzymes (MFE1, TFE), as well as enzymes that hydroxylate prolines of procollagen (collagen prolyl 4-hydroxylases, C-P4Hs). For these studies protein crystallographic approaches are complemented by biophysical characterisations, enzyme kinetics, computing and chemistry. The mechanistic studies concern better understanding of the chemistry of biocatalysis but also of the mechanisms of substrate channelling (MFE1, TFE) and processivity (C-P4H). These studies provide valuable expertise for future biotech and pharmaceutical applications.

Project details

  1. Background and Significance
  2. Recent Progress
  3. Future Goals
  4. Publications 2017-
  5. Research Group Members
  6. National and International Activities

Last updated: 6.7.2018