Light microscopy

Location

Biocenter Oulu Kieppi Laboratories (Aapistie 5A, 4th floor)

Contact information

Veli-Pekka Ronkainen
tel. +358-(0)294 486114, int. 48-6114
e-mail: veli-pekka.ronkainen (at) oulu.fi

User policy, usage instructions and fees

Read the user policy and the information about the fees and usage before making reservations for the first time.

Asimov reservation instructions

Instructions
Asimov reservation page

Equipment

Nikon A1R MP+ multiphoton microscope

Deep and fast imaging of thick samples and small animals in vivo. Dual output infrared laser with tunable (820nm-1300nm) and fixed (1040nm) beams.

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Zeiss LSM 780 laser scanning confocal microscope

For live cell and tissue imaging, sensitive high resolution and spectral imaging of wide range of fluorophores in fixed and living specimens.

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Zeiss Cell Observer spinning disc confocal with FRAP and TIRF

For live-cell imaging, molecular dynamics (FRAP) and cell-surface events detection (TIRF).

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Olympus FluoView FV1000 confocal microscope

Confocal laser scanning microscope for fixed samples.

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OpenSPIM light sheet microscope

For microscopic to mesoscopic scale fixed and living samples. High speed sensitive 4D imaging in 3D culture environment.

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Olympus cellSens time-lapse imaging system

Inverted epifluorescence microscope

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Zeiss upright fluorescence microscope and hyperspectral camera

Upright epifluorescence microscope with normal CCD + optional Rikola/Senop hyperspectral camera.

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Olympus Cell^M imaging system

Upright epifluorescence video microscope, equipped with water dipping objectives. Possibility for FRET.

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IVIS - in vivo imaging system

IVIS Spectrum is an in vivo imaging system for visualization, quantification and 3D reconstruction of both bioluminescent and fluorescent reporters in small animals.

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Analysis software

Bitplane Imaris 3D modelling and analysis software.

Huygens Professional for deconvolution and analysis.

Offline-versions of the microscope control software for data analysis.

Methods

Visualization (3D, 4D) of cells and tissues with light microscopes.

Protein localization - Immunofluorescence microscopy.

Measurements of ion activities and fluorophore signals in living cells.

Long-term cell and tissue culture under a microscope.

Image processing and morphometry.

Tissue stiffness measurements.

Correlative light and electron microscopy (CLEM), in collaboration with EM core facility.

Last updated: 11/4/2017