Zeiss Cell Observer spinning disc confocal microscope

For live-cell imaging, molecular dynamics (FRAP) and cell-surface events detection (TIRF).

Excitation:
405nm, 488nm and 561nm lasers, metal-halide lamp
Detection:
Hamamatsu EMCCD and CCD cameras, fluorescence filters:
Spinning disc: DAPI (BP 450/50nm), GFP (BP 525/50nm) and mCherry (BP 629/62nm)
TIRF: Filter Set 74 HE (DBP 525/31nm + 616/57nm) and Filter Set 76 HE (TBP 459nm + 525nm + 608nm)
EPI: DAPI (BP ex. 359/24nm, em. 445/50nm), GFP (BP ex. 470/20nm, em. 525/50nm), Texas Red (ex. 560/20nm, em. 630/75nm)
Transmitted light detection (DIC 25x-100x objectives/Phase contrast 10x objective)
Spinning disc unit:
Yokogawa CSU-X1
Objectives:
Plan NeoFluar 10x/0.30 Ph1 (air)
LD LCI Plan-Apochromat 25x/0.8 Imm. Korr DIC (water, oil, glycerine)
EC Plan NeoFluar 40x/0.75 DIC (air)
alpha Plan-Apochromat 63x/1.46 Korr DIC (oil)
alpha Plan-Apochromat 100x/1.46 DIC (oil)
objectives from Zeiss LSM 780 on request
Autofocus:
software based or hardware-based DefiniteFocus
Incubator:
Top stage incubator for live cell/tissue time lapse imaging with temperature, humidity and CO2 control
Software:
Zen 2012 (Blue edition)

Total internal reflection fluorescence (TIRF) microscopy with 405nm, 488nm and 561nm excitation

Fluorescence recovery after photobleaching (FRAP), DirectFRAP-module

Fully motorized inverted microscope and stage for multi-position imaging

Suitable sample formats: slides, slide chambers, 35mm and 50mm dishes, 6-well and 24-well plates

Last updated: 13/9/2016