User policy and fees

Access

BCO-TIC light microcopy core facility is open for all users. Facility services include light, fluorescent and confocal microscopy, live-cell imaging, cell and tissue manipulations and measurements, computer-based demonstration and analysis of the image data and morphology. Assistance in sample preparation, in trouble-shooting, choosing the appropriate instruments, and in data analysis is also provided. Imaging instruments and computer rooms for data analysis are located on the fourth floor in the main building of the medical campus (Kieppi building, Aapistie 5A). Cell culture space to culture and manipulate cells before and between microscopic imaging is available. BCO-TIC microscopes and image analysis software are open to all researchers after the training. Training in the BCO-TIC is available by appointment. If there is a need for instruments outside the office hours (16.00-7.30) or on weekends when the building and the corridor doors are locked, the user should contact staff (BCO secretary Anne Vainionpää) to get an electronic guest key.

Reservations

After the training a user account is created and the new user will have access to the reservation system. Imaging instruments are reserved through a web-based Asimov reservation book. By using the same online system, users can revise and cancel their reservations (Instructions). Cancellations should be done as early as possible but at least 1 h before the reservation starts. Note that it is not allowed to use any microscope without a reservation, since the usage will be charged according to Asimov logs. After the imaging session, a user has to log in to confirm actually used hours. Otherwise all reserved hours will be marked as used. The reservations are made in one-hour blocks. Booking time of Olympus FluoView 1000 and Zeiss LSM 780 confocal microscopes is limited to four hours per day per user (during prime time: Mon-Fri, 8.00-16.00).

Charging

Microscope usage time is charged according to the actual hours marked to Asimov after imaging. Usage time must be confirmed to Asimov by the user after the microscopy session. Accumulated invoices will be charged twice / year. There is a maximum expense limit of 4000€ / charging period (two six month periods: 1.1. – 30.6. and 1.7. – 31.12.) / research group, and hours exceeding that limit during that period will not be charged. Usage hours at night between 24.00 and 8.00, user training and support, usage of offline image analysis workstations (room 419A) and cell culture space are free of charge. Customized service packages are formed for industrial users.

Prices for academic users (€/hour)

Bioptonics 3001 optical projection tomography (OPT) device
10€
Leica histology microscope and camera
10€
Olympus cellSens time-lapse imaging system
10€
Olympus Cell^M imaging system
10€
Olympus FluoView FV1000 confocal microscope
15€
Zeiss fluorescence microscope with a microprobe indenter device
10€
Zeiss LSM 780 confocal microscope
15€
Zeiss Cell Observer spinning disc confocal microscope
15€
Nikon multiphoton microscope
15€
IVIS in vivo imaging system
10€
OpenSPIM light sheet microscope
10€
Off-line analysis workstations (Zen, Imaris, Huygens etc.)
0€

Usage

It is not allowed to use microscopes without an instrument-specific training session given by the core facility personnel. Specific instructions and printed user guides are alongside the microscopes. It is not allowed to change the microscope assemblies without negotiating with the staff. Users should inform the staff in the case of malfunction. It is on the last users’ responsibility to turn off the microscopes at the end of the day. Microscope computers have temporary data storage only during the imaging and the user data should be transferred after the experiments. User data can be transferred from the instrument computers using a network drive. Further instructions will be provided by the core facility staff. The network drive is meant for data transfer only, not for personal storage.

Data publication – Acknowledgements

BCO-TIC would greatly appreciate if the light microcopy core facility is acknowledged in the publications when services are provided. We also kindly ask you to send a notification about your BCO-TIC -supported publication(s) to Lauri Eklund (lauri.eklund (at) oulu.fi).

Last updated: 9/2/2017