Ready-to-run sequencing

Ready-to-run sequencing is an option for those who prefer to do sequencing reactions and purifications themselves.

  • Sequencing reactions done with BigDyeTerminator v1.1 cycle sequencing kit (Applied Biosystems)
  • Ready-to-run samples are brought in dried.

Template purification i.e. removal of PCR reaction “residuals” (primers, dNTPs, salt, non-specific PCR products) is crucial for good quality sequence. Depending on the quality of the PCR product methods that can be used are:

  • ExoI/SAP treatment
  • Commercial column purification
  • Gel purification
  • Purification with magnetic beads (Agencourt Ampure or similar)

Last updated: 7.1.2016