Viral Gene Transfer
The BCO Virus Core laboratory provides services in CRISPR gene modification and viral vectors.
All the necessary reagents, consumables and molecular tools are available for retroviral (MuLV), lentiviral and adenoviral gene transfer approaches. We also help in designing custom-made viral RNA interference (RNAi) gene-silencing constructs and CRISPR-based gene KO constructs and in planning genetic manipulation of mouse embryonic stem cells and/or generation of lenti-transgenic mouse models.
The Virus Core laboratory is accessible to trained users at any time. It contains two laminar hoods bookable via Asimov reservation system Laminar 1 and Laminar 2. A high-quality Zeiss inverted fluorescence microscope equipped with a sensitive camera and a standard stereomicroscope are available for imaging. Purification and concentration of viral vectors can be carried out by ultracentrifugation or FPLC chromatography. An Ultra-low-temperature freezer is provided for users to allow storage of viral preparations at the BCO Virus Core laboratory.
Aki Manninen, Ph.D.,
Coordinator of the Virus Core laboratory
Tel. +358-(0)294 486081
E-mail: aki.manninen (at) oulu.fi
Room: 474B, Aapistie 5A
Tel. +358-(0)294 486063
E-mail: mari.sujala (at) oulu.fi
Room: 449B, Aapistie 5A