Some aspects of short pulse laser applications in life sciences

 

 

Some aspects of short pulse laser applications in life sciences

Lecturer: Dr. Artashes Karmenyan, Biophotonics and Molecular Imaging Research Center (BMIRC), National Yang-Ming University, Taipei, Taiwan.

Date: September 30 – October 1, 2014

Place: University of Oulu, Optoelectronics and Measurement Techniques Laboratory, TS 267

Course language: English

Format: Lectures (6 hours) + laboratory demonstrations

Duration of course: 2 days, Lectures on both days at 9-12, Demonstration: September 30, at 13-14,

Final exam: October 1, at 13-14

 

Registration by 26^th September: matti.kinnunen@ee.oulu.fi

Contents

• Introduction - (optical methods in Life Science, Optical pulse techniques (From continue wave to short pulse duration), attempts of realizations, theory, experiments).
• Pulse light after lasers creation – ideas for applications with advantage, experimental realization.                                                                                                             
• Mechanisms of Pulsed Laser nanosurgery of cells and tissues. Ablation of Biological Tissues, Intracellular nanosurgery.  Conditions of use. Experimental results.
• Time resolve spectroscopy, lifetime measurements, Experimental realization of optical time measurements (steady-state, Time-Correlated Single Photon Counting (TCSPC), etc.
• Lifetime imaging, Lifetime Imaging Techniques for Optical Microscopy, (fluorescence lifetime imaging-FLIM), Multi Photon Imaging, Multi Wavelength (TCSPC) Imaging.
• Laser-assisted optoporation of single cells, transfection. Laser-Assisted Optoporation for Drug and Gene Delivery. Laser embryology. Combined use of short pulse lasers - multiphoton imaging and cell surgery, single cell registration by (femtosecond) Optical tweezers and 2p excitation. Other contemporary applications.