Some aspects of short pulse laser applications in life sciences
Lecturer: Dr. Artashes Karmenyan, Biophotonics and Molecular Imaging Research Center (BMIRC), National Yang-Ming University, Taipei, Taiwan.
Date: September 30 – October 1, 2014
Place: University of Oulu, Optoelectronics and Measurement Techniques Laboratory, TS 267
Course language: English
Format: Lectures (6 hours) + laboratory demonstrations
Duration of course: 2 days, Lectures on both days at 9-12, Demonstration: September 30, at 13-14,
Final exam: October 1, at 13-14
Registration by 26th September: firstname.lastname@example.org
- Introduction - (optical methods in Life Science, Optical pulse techniques (From continue wave to short pulse duration), attempts of realizations, theory, experiments).
- Pulse light after lasers creation – ideas for applications with advantage, experimental realization.
- Mechanisms of Pulsed Laser nanosurgery of cells and tissues. Ablation of Biological Tissues, Intracellular nanosurgery. Conditions of use. Experimental results.
- Time resolve spectroscopy, lifetime measurements, Experimental realization of optical time measurements (steady-state, Time-Correlated Single Photon Counting (TCSPC), etc.
- Lifetime imaging, Lifetime Imaging Techniques for Optical Microscopy, (fluorescence lifetime imaging-FLIM), Multi Photon Imaging, Multi Wavelength (TCSPC) Imaging.
- Laser-assisted optoporation of single cells, transfection. Laser-Assisted Optoporation for Drug and Gene Delivery. Laser embryology. Combined use of short pulse lasers - multiphoton imaging and cell surgery, single cell registration by (femtosecond) Optical tweezers and 2p excitation. Other contemporary applications.
Last updated: 12.9.2014