Aki Manninen

Aki Manninen


Coordinator of Cell Biology, Adjunct Prof. of Cell Biology at the Faculty of Biochemistry and Molecular Medicine
Molecular Cell Biology

38 peer reviewed publications in international journals. The author has a h-index of 24.00. (Google Scholar)

Research interests

  • Epithelial polarity, prostate cancer, integrins, extracellular matrix

Publications in peer-reviewed journals

  1. Raykhel I, Moafi F, Myllymäki SM, Greciano PG, Matlin KS, Moyano JV, Manninen A*, Myllyharju J* (2018) Bambi is a novel HIF1-dependent modulator of TGFβ-mediated disruption of cell polarity in hypoxia. J Cell Sci  doi: 10.1242/jcs.210906, Published 23 April 2018, *equal contribution
  2. Uusimaa J, Kaarteenaho R, Paakkola T, Tuominen H, Karjalainen MK, Nadaf J, Varilo T, Uusi- Mäkelä M, Suo-Palosaari M, Ilkka Pietilä I, Hiltunen AE, Ruddock L, Alanen H, Biterova E, Miinalainen I, Salminen A, Soininen R, Manninen A, Sormunen R, Kaakinen M, Vuolteenaho R, Herva R, Vieira P,Dunder T, Kokkonen H,Moilanen JS, Rantala H, Nogee LM, Majewski J, Rämet M, Hallman M,Hinttala R (2018) NHLRC2 variants identified in patients with Fibrosis, Neurodegeneration, and Leptomeningeal Angiomatosis (FINLA) - Characterization of a novel  cerebropulmonary disease. Acta Neuropathologica, 135: 727-742.
  3. Zhang K, Myllymäki SM, Gao P, Devarajan R, Kytölä V, Nykter M, Heljäsvaara R, Wei GH, Manninen A (2017) Oncogenic K-Ras upregulates ITGA6-expression via FOSL1 to induce anoikis resistance and synergizes with αV-class integrins to promote EMT. Oncogene, 36: 5681-5694.
  4. Matlin KS, Myllymäki SM, Manninen A. (2017) Laminins in Epithelial Cell Polarization - Old Questions in Search of New Answers. Cold Spring Harbor Perspectives in Biology, 9: a027920.
  5. Moilanen J, Löffek S, Kokkonen N, Salo S, Väyrynen J, Hurskainen T, Manninen A, Riihilä P, Heljasvaara R, Franzke CW, Kähäri VM, Salo T, Mäkinen M, Tasanen K (2017). Significant role of collagen XVII and integrin β4 in migration and invasion of the less aggressive squamous cell carcinoma cells. Scientific Reports, 7:45057.
  6. Manninen A, Varjosalo M (2017) A proteomics view on integrin-mediated adhesions. Proteomics, 17: p.201600022.
  7. Zhang K, Lee HM, Wei GH, Manninen A (2016) Meta-analysis of gene expression and integrin-associated signaling pathways in papillary renal cell carcinoma subtypes. Oncotarget, 7: 84178-84189
  8. Manninen A (2015) Epithelial polarity - Generating and integrating signals from the ECM with integrins. Exp Cell Res. 334: 337-349
  9. Cattavarayane S, Palovuori R, Jayendrakishore TR, Manninen A. (2015) α6β1- and αV-integrins are required for long-term self-renewal of murine embryonic stem cells in the absence of LIF. BMC Cell Biol. :3
  10. Carrera M, Bitu CC, de Oliveira CE, Cervigne NK, Graner E, Manninen A, Salo T, Coletta RD. (2015) HOXA10 controls proliferation, migration and invasion in oral squamous cell carcinoma. Int J Clin Exp Pathol. 8(4):3613-23
  11. Junttila S, Saarela U, Halt K, Manninen A, Pärssinen H, Lecca RM, Brandli AW, Sims-Lucas S, Skovorodkin I, Vainio SJ. (2014) Functional genetic targeting of the embryonic kidney progenitor cells ex vivo. J. Am. Soc. Nephrol. 26(5):1126-37.
  12. Huang Q, Whitington T, Gao P, Lindberg JF, Yuehong Yang Y,Sun J, Väisänen MR, Szulkin R,Annala M, Yan J, Egevad LA, Zhang K, Lin R, Jolma A, Nykter M, Manninen A, Wiklund F, Vaarala MH, Visakorpi T, Xu J, Taipale J, Wei GH. (2014) A prostate cancer susceptibility allele at 6q22 increases RFX6 expression by modulating HOXB13 chromatin binding. Nature Genetics 46:126–135.
  13. Teräväinen TP, Myllymäki SM, Friedrichs J, Strohmeyer N, Moyano JV, Wu C, Matlin KS, Muller DJ, Manninen A. (2013) αV-integrins are required for mechanotransduction in MDCK epithelial cells. PLoS ONE, 8(8):e71485.
  14. Rilla K, Pasonen-Seppänen S, Kärnä R, Karjalainen H, Törrönen K, Koistinen V, Tammi MI, Tammi RH, Teräväinen T, Manninen A. (2012) HAS3-induced accumulation of hyaluronan in 3D MDCK cultures results in mitotic spindle misorientation and disturbed organization of epithelium.
  15. Greciano PG, Moyano JV, Buschmann MM, Tang J, Lu Y, Rudnicki J, Manninen A, Matlin KS. (2012) Laminin 511 Partners with Laminin 332 to Mediate Directional Migration of Madin-Darby Canine Kidney (MDCK) Epithelial Cells. Mol Biol Cell. 23(1):121-36.
  16. Veikkolainen V, Naillat F, Railo A, Chi L, Manninen A, Hohenstein P, Hastie N, Vainio S, Elenius K. (2012) ErbB4 signaling regulates cell division and polarization during kidney epithelial development. J. Am. Soc. Nephrol. 23(1):112-22.
  17. de Oliveira JT, de Matos AJ, Santos AL, Pinto R, Gomes J, Hespanhol V, Chammas R, Manninen A, Bernardes ES, Reis CA, Rutteman G, Gärtner F. (2011) Sialylation regulates galectin-3/ligand interplay during mammary tumour progression: a case of targeted uncloaking Int. J. Dev. Biol. 55(7-8-9):823-834.
  18. Myllymäki SM, Teräväinen TP, Manninen A. (2011) Two Distinct Integrin-Mediated Mechanisms Contribute to Apical Lumen Formation in Epithelial Cells. PLoS ONE 6(5): e19453.
  19. Moilanen AM, Rysä J, Mustonen E, Serpi R, Aro J, Tokola H, Leskinen H, Manninen A, Levijoki J, Vuolteenaho O, Ruskoaho H. (2011) Intramyocardial BNP Gene Delivery Improves Cardiac Function Through Distinct Context-Dependent Mechanisms. Circ Heart Fail. 4:483-95.
  20. de Oliveira JT, de Matos AJ, Gomes J, Vilanova M, Hespanhol V, Manninen A, Rutteman G, Chammas R, Gärtner F, Bernardes ES. (2010) Coordinated expression of galectin-3 and galectin-3-binding sites in malignant mammary tumours: implications for tumour metastasis. Glycobiology, 20:1341-52.
  21. Friedrichs J, Manninen A, Muller DJ, Helenius J. (2008) Galectin-3 regulates integrin α2β1-mediated adhesion to collagen-I and ­IV.  J. Biol. Chem. 283: 32264-72.
  22. Arpiainen S, Järvenpää S-M, Manninen A, Viitala P, Lang MA, Pelkonen O, Hakkola J. (2008) Coactivator PGC-1 regulates the fasting inducible xenobiotic-metabolizing enzyme CYP2A5 in mouse primary hepatocytes. Toxicol. Appl. Pharm. 232:135-41.
  23. Torkko JM, Manninen A, Schuck S, Simons K. (2008) Depletion of apical transport proteins perturbs epithelial cyst formation and ciliogenesis. J. Cell Sci. 121:1193-203.
  24. Friedrichs J, Torkko JM, Helenius J, Teräväinen TP, Fullekrug J, Muller DJ, Simons K, Manninen A. (2007) Contributions of galectin-3 and -9 to epithelial cell adhesion analysed by single cell force spectroscopy. J. Biol. Chem. 282: 29375-83.
  25. Schuck S, Gerl MJ, Ang A, Manninen A, Keller P, Mellman I, Simons K. (2007) Rab10 is involved in basolateral transport in polarized Madin-Darby canine kidney cells. Traffic 8:47-60.
  26. Manninen A, Verkade P, Le Lay S, Torkko J, Kasper M, Fullekrug J, Simons K. (2005) Caveolin-1 is not essential for biosynthetic apical membrane transport. Mol. Cell. Biol. 25:10087-10096.
  27. Vieira O, Verkade P, Manninen A, Simons K. (2005) FAPP2 is involved in the transport of apical cargo in polarized MDCK cells. J. Cell. Biol. 170:521-526.
  28. Delacour D, Gouyer V, Zanetta J-P, Drobecq H, Leteurtre E, Grard G, Moreau-Hannedouche O, Maes E, Pons A, Andre S, Le Bivic A, Gabius HJ, Manninen A, Simons K, Huet G. (2005) Galectin-4 and sulfatides in apical membrane trafficking in enterocyte-like cells. J. Cell. Biol. 169:491-501.
  29. Schuck S*, Manninen A*, Honsho M, Fullekrug J, Simons K. (2004) Generation of single and double knockdowns in polarized epithelial cells by retrovirus-mediated RNA interference. Proc. Natl. Acad. Sci. USA 101:4912-4917.*equal contribution.
  30. Sillanpaa N, Magureanu CG, Murumagi A, Reinikainen A, West A, Manninen A, Lahti M, Ranki A, Saksela K, Krohn K, Lahesmaa R, Peterson P. (2004) Autoimmune regulator induced changes in the gene expression profile of human monocyte-dendritic cell-lineage. Mol Immunol 41(12):1185-98
  31. Lahti AL, Manninen A, Saksela K. (2003) Regulation of T cell activation by HIV-1 accessory proteins: Vpr acts via distinct mechanisms to cooperate with Nef in NFAT-directed gene expression and to promote transactivation by CREB. Virology 310(1):190-6
  32. Manninen A., Saksela K. (2002) HIV-1 Nef interacts with IP3R to activate calcium signaling in T cells. J Exp Med 195(8):1023-32
  33. Hiipakka, M., Huotari, P., Manninen, A., Renkema, G.H., and Saksela, K. (2001) Inhibition of cellular functions of HIV-1 Nef by artificial SH3 domains. Virology 286: 152-9
  34. Manninen A., Huotari P., Hiipakka M,. Renkema G-H., Saksela K. (2001) Activation of NFAT-dependent gene expression by Nef: conservation among divergent Nef alleles, dependence on SH3-binding and membrane association, and cooperation with protein kinase C-q. J Virol 75 (6): 3034-3037
  35. Renkema G-H., Manninen A., Saksela K. (2001) Human immunodeficiency virus type-1 Nef selectively associates with a catalytically active subpopulation of p21-activated kinase 2 (PAK2) independently of PAK2 binding to Nck or b-PIX. J Virol 75 (5): 2154-2160
  36. Manninen A., Renkema G-H., Saksela K. (2000) Synergistic activation of NFAT by HIV-1 Nef and the Ras/MAPK pathway. J Biol Chem 275 (22): 16513-16517
  37. Renkema G-H., Manninen A., Mann D.A., Harris M., Saksela K. (1999) Identification of the Nef-associated kinase as p21-activated kinase 2. Curr Biol 9: 1407-1410
  38. Manninen A., Hiipakka M., Vihinen M., Lu W., Mayer B., Saksela K. (1998) SH3-domain binding function of HIV-1 Nef is required for association with a PAK-related kinase. Virology 250: 273-282


Thesis supervisions

Ph.D Thesis supervision
  • Satu-Marja Myllymäki (Ph.D.) 1.10.2015

  • Sandhanakrishnan Cattavarayane (Ph.D.) 13.5.2016

  • Kai Zhang (Ph.D.) 16.6.2017