HBS-DP Course on Macromolecular Small Angle X-ray and Neutron Scattering (SAXS/SANS)

This practical course addresses the principles and applications of small angle X-ray and neutron scattering (SAXS/SANS) for biological macromolecules. The course constitutes lectures as well as hands-on experience for analyzing the SAXS/SANS data.

Event information

Time

-

Venue location

F101

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Course description:

This practical course addresses the principles and applications of small angle X-ray and neutron scattering (SAXS/SANS) for biological macromolecules. The course is mainly intended for PhD students and postdoctoral researchers who are using or will be using SAXS/SANS for biological macromolecules in their research work. Use of SAXS/SANS for membrane proteins will also be discussed. The course constitutes lectures as well as hands-on experience for analyzing the SAXS/SANS data. The course is organized with the support of the Health and Biosciences Doctoral Program (HBS-DP) of the University of Oulu.

There is a possibility for the selected participants to send a protein sample to DLS directly for SAXS data collection. The beamline scientists will collect SAXS data at the beamline B21 prior to the course (on Oct 19-20, 2021).

The course will be organized as a hybrid event where online as well as on-site participation is possible. For online participation, a computer/laptop is needed. Further details of optimal computer requirements and software that should be installed will be communicated prior to the course. For on-site participation, lecture hall F101A (for lectures) and computer classroom F132 (for hands-on training) at FBMM premises will be used.

Practical sessions are limited to registered participants while lectures are open to a wider audience.

Apply online here! before 25th Sep 2021. There is no registration fee for the course.

Confirmed teachers:
Dr. Nathan Cowieson, Diamond Light Source, UK
Dr. Robert Rambo, Diamond Light Source, UK
Prof. Jan Skov Pedersen, Aarhus University, Denmark
Dr. Anne Martel, Institut Laue-Langevin, France
Dr. Nicolai Tidemand Johansen, University of Copenhagen, Denmark

Credits:
1.0 ECTS

Contact Persons:
Rajaram Venkatesan & Pia Askonen (firstname.lastname@oulu.fi)

Organizer:
Rajaram Venkatesan, Faculty of Biochemistry and Molecular Medicine, University of Oulu

Course Programme:

17th November 2021, Wednesday

09:00-09:15 Registration and coffee (F101)
09:15-12:30 Session 1: Lectures (Open to everyone)
09:15-09:20 Rajaram Venkatesan; Opening remarks
09:20-10:00 Nathan Cowieson; SAXS introduction and applications
10:00-10:15 Nikul Kunti; B21-beamline access
10:15-11:00 Robert Rambo; Modern bioSAXS theory and basics
11:00-11:45 Anne Martel; BioSANS introduction, neutron specificities
11:45-12:30 Anne Martel; Sample environment, data collection and initial analysis
12:30-13:30 Lunch
13:30-18:00 Session 2: Practicals (Registered participants only)
13:30-14:30 Attendees, Attendee Introduction/Presentation, 2 slides per user explaining their SAXS/SANS project
14:30-15:30 Team Diamond, Data reduction, data quality assessment and primary analysis (tutorial)
15:30-15:45 Break
15:45-18:00 Team Diamond; Modelling tutorial (user or example data)

18th November 2021, Thursday

09:00-12:15 Session 3: Lectures (Open to everyone)
09:00-09:45 Jan Skov Pedersen; Absolute scale modelling of SAXS: Micelles, proteins and
rigid body refinement
09:45-10:30 Jan Skov Pedersen; SAXS for membrane proteins: Modelling and refinement
10:30-10:45 Break
10:45-11:30 Robert Rambo; Developments in volumetric (ab initio) modelling
11:30-12:15 Nicolai Tidemand Johansen; SANS for membrane proteins: sample considerations
12:15-13:30 Lunch
13:30-17:30 Session 4: Practicals (Registered participants only)
13:30-15:00 Jan Skov Pedersen; Demonstration with Jan’s program: Absolute scale modelling
15:00-15:15 Break
15:15-17:30 Anne Martel/ Nicolai Tidemand Johansen; SANS demonstration with user data

19th November 2021, Friday

09:00-10:30 Session 5: Lectures (Open to everyone)
09:00-09:45 Nathan Cowieson; Hybrid and atomistic modelling
09:45-10:30 Nicolai Tidemand Johansen; Examples of SANS for membrane proteins
10:30-10:45 Break
10:45-17:30 Session 6: Practicals (Registered participants only)
10:45-12:15 & 13:15-15:30 Team Diamond; Modelling tutorial (user or example data)
12:15-13:15 Lunch
15:30-17:00 All teachers! User data: Preparation/Presentation of results
17:00-17:30 General discussion/Feedback

Last updated: 9.11.2021