Collagen prolyl 4-hydroxylase

In this project we try to unravel the reaction mechanism of collagen prolyl 4-hydroxylase (C-P4H). The substrate of C-P4H is procollagen, having the (XYG) repeat. Many of the X and Y residues are prolines and many of the Y prolines are hydroxylated by C-P4H. In particular we are interested in understanding the processivity properties of C-P4H.
This figure visualises the current model of the C-P4H tetramer

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Other Finnish

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University of Oulu

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Project description

This is a multidisciplinary project, together with research groups at University of Oulu, the University of Eastern Finland and the University of Pavia, Italy. A wide range of techniques is used, including protein crystallography, cryoEM, mass spectrometry, enzymology.