Single-cell RNA-seq data analysis using Chipster
This hands-on online course introduces single-cell RNA-seq (scRNA-seq) data analysis methods. It covers the processing of transcript counts from quality control and filtering to dimensional reduction, clustering, cell type identification and cluster marker gene detection. You will also learn how to do integrated analysis of multiple samples.
Time: January 24-25, 2024. Both days 9:00-12:30.
Place: Online (Zoom)
Credits: 1 ETCS
Practicalities: The course consists of lectures and exercises. The lectures will be pre-recorded, and participants are requested to view the videos prior to the course and test their knowledge with a set of questions. This gives you more time to reflect on the concepts so that you can use the classroom time more efficiently for discussions and exercises.
Prerequisities: In the exercises, we use Seurat tools embedded in the free and user-friendly Chipster software. No experience in R is required and the course is thus suitable for everybody who is planning to use single-cell RNA-seq.
Trainers: Maria Lehtivaara, Eija Korpelainen and Ellimari Paunio (CSC)
Content: You will learn how to
- perform quality control and filter out low quality cells
- normalize gene expression values
- remove unwanted sources of variation
- select highly variable genes and perform dimensionality reduction (PCA)
- cluster cells
- visualize clusters using UMAP and tSNE
- identify cell types using reference-based SingleR
- find marker genes for a cluster
- integrate multiple samples
- find conserved cluster marker genes for two samples
- find genes which are differentially expressed between two samples in a cell type specific manner
- visualize genes with cell type specific responses in two samples
Registration: Register for the course at Peppi (code DR00AR92-3001) at the latest on 12th of January 2024. Priority is given to doctoral researchers. The number of participants is limited to 30.