Biocenter Oulu Sequencing Center

Information about the service:

For sequencing of multiple targets at the same time, we offer next –generation sequencing using two different platforms: Illumina’s NextSeq550 and Ion Torrent.

More information about the service.

Information about the service:

Capillary sequencing is offered with ABI3500xL Genetic Analyzer-system.

More information about the service.

Information about the service:

• Isolation is done with QIAsymphony SP instrument
• The purity and concentration of isolated DNA is determined spectrophotometrically
• For further information, please contact: Annika Väntänen, tel. +358-(0)294 48 5752 or annika.vantanen (at) oulu.fi, lbkseq (at) oulu.fi

Information about the service:

• Automated pipetting can be done with QIAgility, which is a compact benchtop instrument offering rapid and reliable pipetting
• Examples of applications: Real-time RT-PCR setup, normalization, serial dilution, restriction digestion and PCR setup. Explore the virtual demo to learn more
• Reservations using the Asimov system. New users! Before the first reservation contact the core facility for training.
• Located in Aapistie 5, Rm 276B
• Price: 3.0 € per hour (~ plate). Customer provides the plastics.
• Contact: lbkseq (at) oulu.fi

Information about the service:

• Automated electrophoresis (sizing, quantitation and quality control) is done with Agilent 2100 Bioanalyzer system
• Customer delivers diluted aliquots of DNA/RNA samples
• Eleven/twelve samples can be processed with one chip
• Contact: ngs (at) oulu.fi

Pricing info

Prices are indicated as VAT 0%

Service request form

Bioanalyzer form

Details and specifications for Agilent 2100 bioanalyzer assays

Samples have to be delivered in a suitable buffer and concentration.

Following kits are available:

Agilent High Sensitivity DNA Kit

Separation, sizing and quantification of low concentrated dsDNA samples from 50 to 7000 bp.

%CV= coefficient of variance

Agilent DNA 1000 Kit

For the separation, sizing and quantification of dsDNA fragments from 25 to 1000 bp.

%CV= coefficient of variance

Agilent RNA 6000 Nano Kit

For the analysis and quantitation of total and mRNA samples of 25 to 500 ng/µL in concentration.

%CV= coefficient of variance

Information about the service:

• BioRad (CFX96 Touch™ Real-Time PCR Detection System) is offered for use as self-service
• Reservations can be made through iLab
• Located in Aapistie 5, Rm 444B
• Usage price 1.2 € per hour
• Contact: lbkseq (at) oulu.fi

If unfamiliar with the Biorad CFX96 Touch™ Real-Time PCR Detection System, ask for introduction from the core personnel.

Plates:

• Use only low-profile plates! Using other plates will harm the lid system.
• To avoid evaporation of samples it is recommended to use skirted plates
• Plates recommended (Bio-Rad) low-profile, skirted: HSP-9601, HSP-9655, HSP9631, HSP9901, HSP9955, HSP9611, HSP9621, HSP9635, HSP9641, HSP9645, HSP9661, HSP9665, HSP9666, HSP9601B

If there are any problems or you get error messages, record them in the booklet and inform the personnel.