PCR product and plasmid sequencing
Information about the service
- Capillary sequencing is performed with ABI3500xL Genetic Analyzer
- Sequencing reactions are done with BigDyeTerminator v1.1 cycle sequencing kit (ABI) and purified by ethanol precipitation
- Customer delivers purified DNA sample combined with appropriate primers
- Contact: lbkseq (at) oulu.fi
DNA sequencing services are located at the Biocenter Oulu facilities, Medical Campus, Aapistie 5A 90220 Oulu, Rm 448B.
Please, read the following instructions carefully, since samples without adequate information will not be processed.
Internal customers – iLab for submitting sequencing request
External customers – sequencing form (found in “forms” section)
Samples are placed in the cold room 442B and must be accompanied with an iLab request (internal customers) or a fully filled sequencing form (external customers, place in a box near room 448B or send via e-mail).
Samples will be run in the order of arrival and should be brought in before 3 pm to be run during the next working day. Results can be obtained from server within three (3) working days.
For non-OULU University/non-UNIV users: e-mail only.
Due to the large number of researchers we have no capacity to perform sequencing reactions for individual annealing temperatures. We provide three annealing temperatures: 50°C, 55°C, and 60°C. Please take this into consideration when designing your primers.
Sample list should be provided as an Excel-file via iLab (external customers: lbkseq (at) oulu.fi). Excel-file name has to include owner’s name and date.
There is no limit for the size of the sample name, but they should NOT include Scandinavian letters, spaces or any of the following:
\ /: * ? " ‘ , < > |
Sample names should start with your initials (for example MM_1_DN13_COL1A2_e5F).
NOTE: To avoid mistakes in the analysis, please do not use the whole sample name when marking individual tubes. Your initials and running number in tubes relative to your excel file is sufficient and more easily readable.