Location
Biocenter Oulu Kieppi Laboratories (Aapistie 5A, 4th floor)
Contact information
Veli-Pekka Ronkainen
tel. +358-(0)294 486114, int. 48-6114
e-mail: veli-pekka.ronkainen (at) oulu.fi
User policy, usage instructions and fees
Read the user policy and the information about the fees and usage before making reservations for the first time.
Asimov reservation instructions
Instructions
Asimov reservation page
Data publication – Acknowledgements
BCO-TIC would greatly appreciate if the light microcopy core facility is acknowledged in the publications when services are provided. We also kindly ask you to send a notification about your BCO-TIC -supported publication(s) to Lauri Eklund (lauri.eklund (at) oulu.fi).
Here you can find an example on how to Acknowledge Biocenter Oulu Research Infrastructure.
Part of the Biocenter Finland biological imaging platform services
A member of Euro-BioImaging through Finnish advanced light microscopy node
Equipment
Leica SP8 FALCON laser scanning confocal microscope
For high resolution and speed imaging of living and fixed samples. A white light laser and spectral detectors for most available fluorophores. Fast lifetime contrast (FLIM) imaging and real time adaptive deconvolution.
Nikon A1R MP+ multiphoton microscope
Deep and fast imaging of thick samples and small animals in vivo. Dual output infrared laser with tunable (820nm-1300nm) and fixed (1040nm) beams.
Zeiss LSM 780 laser scanning confocal microscope
For live cell and tissue imaging, sensitive high resolution and spectral imaging of wide range of fluorophores in fixed and living specimens.
Zeiss Cell Observer spinning disc confocal with FRAP and TIRF
For live-cell imaging, molecular dynamics (FRAP) and cell-surface events detection (TIRF).
Olympus FluoView FV1000 confocal microscope
Confocal laser scanning microscope for fixed samples.
OpenSPIM light sheet microscope
For microscopic to mesoscopic scale fixed and living samples. High speed sensitive 4D imaging in 3D culture environment.
Olympus cellSens time-lapse imaging system
Inverted epifluorescence microscope
Zeiss Axio Imager motorized histology microscope
A fully motorized microscope with a color camera. Possibility for automated high resolution imaging of large samples.
Zeiss upright fluorescence microscope and hyperspectral camera
Upright epifluorescence microscope with normal CCD + optional Rikola/Senop hyperspectral camera.
Olympus Cell^M imaging system
Upright epifluorescence video microscope, equipped with water dipping objectives. Possibility for FRET.
IVIS - in vivo imaging system
IVIS Spectrum is an in vivo imaging system for visualization, quantification and 3D reconstruction of both bioluminescent and fluorescent reporters in small animals.
Analysis software
- Bitplane Imaris 3D modelling and analysis software.
- Huygens Professional for deconvolution and analysis.
- Offline-versions of the microscope control software for data analysis.
Methods
- Visualization (3D, 4D) of cells and tissues with light microscopes.
- Protein localization - Immunofluorescence microscopy.
- Measurements of ion activities and fluorophore signals in living cells.
- Long-term cell and tissue culture under a microscope.
- Image processing and morphometry.
- Tissue stiffness measurements.
- Correlative light and electron microscopy (CLEM), in collaboration with EM core facility.
Last updated: 19.3.2020