The Jianan Lab of Nanophotonic Biosensors

Research group information

Unit and faculty

Research Unit of Health Sciences and Technology

Faculty of Medicine

Disease Networks

Faculty of Biochemistry and Molecular Medicine

Contact information

Research group leader

Tenure-track Assistant Professor

Jian-An Huang

Researchers

Research group description

The Jianan lab develops optical nanotechnologies for label-free studies of single molecules, single particles and single cells by combining molecular manipulation, plasmonic nanostructures and plasmon-enhanced spectroscopy towards wearable healthcare and personalized medicine.

Projects

Project

2026 – 2029

RamanProSeq: Plasmonic nanopore technologies for automatic manipulation and Raman spectroscopic sequencing of single proteins

Project

2025 – 2028

Next generation molecular sensing (NGSens)

Project

2024 – 2027

Digital Raman assay for single-molecule protein sequencing in single live cells (LiveProSeq)

Project

2023 – 2024

A wearable Raman sensor for monitoring metabolic secretions in sweat

Project

2023 – 2026

Label-free single-molecule digital Raman assay for quantification of low abundance biomarkers (DigiRaman)

Project

2022 – 2027

Plasmonic two-nanopore technology for single-molecule protein sequencing (TwoPoreProSeq)

Project

2022 – 2024

Wearable Lab-in-a-watch Optical Sensor for Non-invasive Sweat Diagnosis

Research

Single-molecule detection and sequencing

Wearable Lab-in-a-Watch optical sensor

Single-cell delivery, extraction and analysis

Single-cell analysis provides important information of biology that are masked in analysis of cell populations and tissues. Current single-cell analysis techniques allow cell analysis at the genomic, transcriptomic and proteomic levels but have to lyse the cells to extract the cytosolic contents. Thus, it hampers real-time monitoring of intracellular dynamic transformations, such as differentiation and gene expression of single living cells, and, more importantly, interconnection between living cells with time. What is worse, the amount of extracted molecules from single cell were too little to be analyzed by conventional methods such as mass spectrometery.

The research goal of this topic is to develop optical biosensing methods using surface-enhanced Raman spectroscopy (SERS) as the sensing function for single-molecule and real-time single-cell analysis. A hollow nanoelectrode system is used to electroporate cell membrane, after which molecules or nanoparticles can be delivered into cytosol.[1] Alternatively, It can extract and sample intracellular content from specific cell into the microfluidic channel by electrical bias.[2] The extract can be detected by the plasmonic nanopores for single-molecule SERS characterization of the intracellular content.[3] By cycling sampling and detection, the integrated system will find broad applications for different cellular systems, such as cancer cells response to drugs.

Related works:

J.A. Huang et al., On-Demand Intracellular Delivery of Single Particles in Single Cells by 3D Hollow Nanoelectrodes, Nano letters 19 (2), 722–731 (2019)
Caprettini, Valeria, et al. "Enhanced Raman investigation of cell membrane and intracellular compounds by 3D plasmonic nanoelectrode arrays." Advanced Science 5.12 (2018): 1800560.
Huang, Jian‐An, et al. "Multiplexed discrimination of single amino acid residues in polypeptides in a single SERS hot spot." Angewandte Chemie International Edition 59.28 (2020): 11423-11431.

Openning

Current openning of postdoctoral researcher by 8 March 2026

Applications are invited for a full-time

Postdoctoral Researcher in development of plasmonic nanopore for single molecule sequencing by surface enhanced Raman spectroscopy (SERS)

position under supervision by Assistant Professor Jian-An Huang who has strong expertise in single-molecule SERS analysis of biomolecules and plasmonic nanopore fabrication in the Research Unit of Health Science and Technology, Faculty of Medicine at University of Oulu, Finland.

Project description

The postdoctoral researcher will work in the EIC Pathfinder OPEN project: “Plasmonic nanopore technologies for automatic manipulation and Raman spectroscopic sequencing of single proteins (RamanProSeq)” that aims to develop Raman spectroscopic single-molecule protein sequencing technology capable of reading protein primary sequence at single-amino-acid resolution by integrating plasmonic nanopores with a high-speed Raman detection system, an automated control system, computer simulations, and advanced Raman-based bioinformatics.

The RamanProSeq consortium includes Prof. Shuai Li in University of Oulu (Finland), Dr. Francesco De Angelis in Italian Institute of Technology (Italy), Dr. Cuifeng Ying in the Nottingham Trent University (UK), Dr. James Yates in Universidade NOVA de Lisboa (Portugal), Dr. Lu Cheng in University of Eastern Finland (Finland) and Prof. Maria Fyte in RWTH Aachen University (Germany) who are leading experts in intelligent automation, ultrafast SERS spectroscopy, nanopore fabrication and engineering, single molecule biochemistry and biophysics, computational biotechnology and AI bioinformatics. Assistant professor Jianan Huang will be the supervisor for fabrication, development and Raman spectroscopic measurement of plasmonic nanopores on nanopipettes in this call and get support from all the partners.

Description of the position

The tasks of the postdoctoral researcher will be fabrication and characterization of plasmonic nanopore on glass nanopipettes as well as measurement of ion currents and Raman spectroscopy of the biomolecules (DNA, peptides, proteins) in the nanopores such as:

Design and fabricate glass nanopipettes and characterise them by biomolecule translocation;
Design, fabricate and characterize plasmonic nanopores on the nanopipettes with low electric noise;
Measurement and preliminary analysis of ion current and Raman spectra of biomolecules in the device;
The work duties may include teaching with no more than 10% of working time.

The nanopore fabrications can be firstly based on published protocols and then improved to fit the needs of the project. The biomolecule design, molecule-nanopore interfaces, automatic control of molecule translocation in the nanopore and deep data analysis will be handled by other researchers, discussed and solved together in the consortium.

You will work together with our multidiciplinary team, and have access to the state-of-the-art micro- and nanofabrication and characterization facilities, and biological facilities.

What we offer?

Exciting research project and excellent multidisciplinary research environment.
The support of an experienced and enthusiastic team and broad scientific network where you can share your expertise and grow as an expert.
Development and career options of the big organization.
Faculty of Biochemistry and Molecular Medicine and Faculty of Medicine are highly international (~50% of the roughly 200 employees at the faculty are international). University of Oulu has put a great effort towards building a cutting-edge research infrastructure that includes state-of-the-art instrumentation for imaging, gene-modified mice, X-ray crystallography, and quantitative biochemistry. The teams have extensive collaborative national and international networks including Biocenter Finland and the ESFRI projects Instruct, EuroBioimaging, and Infrafrontier.
Wellness benefit ePassi covering sport, culture and well-being. Read more about other staff benefits here.
Our Buddy Programme and Spouse Network support you and your close ones in settling into Oulu.
The support of an experienced and enthusiastic team where you can share your expertise and grow as an expert.
Development and career options of the big organization.
We have an HR Excellence in Research -quality label which is a recognition awarded by the European Commission for the development of researchers’ working conditions and careers.
Work that matters and a workplace that promotes flexibility and work-life balance. Read more about working with us.
Finland is one of the most livable countries in the world, with a high quality of life, safety, an excellent education system, and competitive economy. Read more about living in Oulu.

What we expect from you?

We are looking for an ambitious, highly motivated candidate who has enthusiasm and ability to work both independently and as a team member to tackle challenging problems to achieve common goals.
Doctoral degree in Physics, Electronic Engineering, Materials Science or related field.
Strong experience in micro-/nanofabrication approaches, such as nanoparticle assembly, nanoporous plasmonic materials or cleanroom equipment (e.g. Focused Ion Beam, photolithography, physical vapor deposition), as demonstrated in publications are essential.
Experience in patch-clamp amplifier measurement for nanopore resistive pulse sensing are essential.
Experience in plasmonic or SERS sensing will be considered a plus.
Ability to fluently communicate in English. Both spoken and written English skills are required.
While your main task is research, we expect you to contribute to group management, teaching and supervision of graduate students in Jianan group.

Salary

The position is fixed-term for two years as of 1^st of May 2026 or as soon as possible thereafter.
The salary will be based on the levels 5-6 of the demand level chart for teaching and research staff of Finnish universities. In addition, a salary component based on personal work performance will be paid (maximum of 50 % of the job-specific component). Starting gross salary will be approx. 3600-4000 € per month (before taxes) depending on qualifications and experience.
A trial period of 6 months is applied for the position.

How to apply?

Interested? If yes, please apply by the 8^th of March 2026 (23:59 Finnish local time) through our recruitment system.

The application should be written in English and include the following:

Cover letter shortly describing your motivation to apply for this position
CV following the guidelines of the Finnish Advisory Board on Research Integrity
List of publications, following the guidelines of the Research Council of Finland.
Contact information of at least two persons available for recommendation.

The eligible applicants fitting best in the profile expected for the position will be invited to an on-site or remote interview. All applicants will be notified during the selection process.

Contact Information

If you have any further questions, please contact Dr. Jian-An Huang, email: Jianan.huang@oulu.fi.

Funding opportunity to join us as researchers:

Marie Skłodowska-Curie Actions (MSCA) Postdoctoral Fellowships: Competitive EU fellowships for postdoctoral researchers;
Academy Research Fellow: salaries and research cost for the early-career researcher to build his/her research team.

News

19 - 21.01.2026: #Conference# Jianan presented and discuss our single-molecule SERS research at Oulu (European Capital of Culture 2026) with scientists at the Single-molecule Sensors and nanoSystem conference 2026 (S3IC 2026) at the artistic atmosphere of Rome.

2025

19.12.2025: #Publication# Congratulation for Cindy's publication of Nitrilotriacetic acid functionalized gold nanopillars enable stochastic detection and deep learning analysis of prolines and hydroxyprolines by surface enhanced Raman spectroscopy on Nanoscale.
03.12.2025: #Publication# Congratulation for Ling's publication of Portable and Label-free Optical Detection of Sweat Glucoses using Functionalized Plasmonic Nanopillar Array on Microsystems & Nanoengineering.
25-28.11.2025: #Conference# Jianan join the 23rd Chinese National Conference of Light Scattering at Xiamen University, China, and gave a invited talk: Construction of single plasmonic hot spot towards single-molecule Raman sequencing.
19.10.-4.11.2025: #Visit# Prof. Jian Ye's doctoral student, Mr. Zongyu Wu, from Shanghai Jiaotong University, Shanghai, China visited our group for the project of single-molecule analysis of metabolites in biofluids under the Academy mobile funding 2024.
19-24.10.2025: #Visit# Prof. Jian Ye and Dr. Zhou Chen from Shanghai Jiaotong University, Shanghai, China visited our group for the project of single-molecule analysis of metabolites in biofluids. Prof. Ye gave a BCO seminar talk: “Single-molecule Raman detection of metabolites and toxins” on 23.10.2025.
15.10.2025: #Grant# Congratulation for Jianan to receive the EIC Pathfinder OPEN funding: RamanProSeq together with collaborators from the University of Eastern Finland, Universidade Nova de Lisboa (Portugal), RWTH Aachen University (Germany), Istituto Italiano di Tecnologia (Italy) and Nottingham Trent University (United Kingdom).
08.10.2025: #Publication# Congratulation for Peilin's publication of Single-Molecule Monitoring of Nucleic Acid Dynamics Using Raman Correlation Spectroscopy in Plasmonic Nanowells on ACS Nano.
08.08.2025: #Publication# Congratulation for Mulusew's publication of Single-Molecule SERS Detection of Phosphorylation in Serine and Tyrosine Using Deep Learning-Assisted Plasmonic Nanopore on The Journal of Physical Chemistry Letters.
11.7.2025: #Visit# Jian-An visited Dr. Jinqing Huang's group Hong Kong Univesity of Science and Technology, Hong Kong, China, for the colloabration of single-molecule manupulation and SERS detection.
16.6.-10.7.2025: #Visit# Jian-An visited Prof. Jian Ye's group in Shanghai Jiaotong University, Shanghai, China for the project of single-molecule analysis of metabolites in biofluids under the Academy mobile funding 2024.
16.6.-4.7.2025: #Visit# Yuge visited Dr. Francesco De Angelis' group at from Italian Institute of Technology, Genova, Italy for 3-week research of single-cell SERS analysis.
26.05.2025: #Welcome# Welcome Dr. Ibrar Alam to join our group as a postdoctoral researcher for single-molecule Raman sequencing and single-cell analysis in the NGSens project!
17.04.2025: #Publication# Congratulation for Yingqi's publication of Single-Molecule SERS Discrimination of Proline from Hydroxyproline Assisted by a Deep Learning Model on Nano Letters.
03.04.2025: #Grant# Congratulation for Jianan to receive the Academy mobile funding with China 2024 to collaborate with Prof. Jian Ye in Shanghai Jiaotong University, Shanghai, China on single-molecule analysis of metabolites in biofluids.
12.03.2025: #Grant# Congratulation for Jianan to receive the Proof-of-Concept funding 2025 for wearable biochips together with Prof. Shuai Li in Faculty of Information Technology and Electrical Engineering for wearable sweat stimulation and sensing.
25. - 28.02.2025: #Visit# Mr. Dongbao Tang, a PhD student from Prof. Linbao Luo's group from Hefei University of Technology visited our group for the research of automatic control electronics for single molecule sequencing.
20.11.2024 - 21.02.2025: #Visit# Mulusew visited Prof. Linbao Luo's group from Hefei University of Technology, China for 3 months for the research of automatic control electronics for single molecule sequencing.
01.12.2024 - 28.02.2025: #Visit# Alireza visited Prof. Kang Wang's group at Nanjing University, China for 3 months for the research of 2-pore nanopipettes for single molecule sequencing.

Alumni

Publication

Peer-review articles

2026

Portable and label-free optical detection of sweat glucose using functionalized plasmonic nanopillar array, Ling Liu, Kuo Zhan*, Joni Kilpijärvi, Matti Kinnunen, Yingqi Zhao, Yuan Zhang, Mulusew W. Yaltaye, Yang Li, Artem Zhyvolozhnyi, Anatoliy Samoylenko, Seppo Vainio, and Jianan Huang*, Microsystems & Nanoengineering 2026 12, 43. https://doi.org/10.1038/s41378-025-01152-6
Nitrilotriacetic acid functionalized gold nanopillars enable stochastic detection and deep learning analysis of prolines and hydroxyprolines by surface enhanced Raman spectroscopy, Yuan Zhang, Kuo Zhan, Peilin Xin, Yingqi Zhao, Ibrar Alam, Shubo Wang, Aliaksandr Hubarevich, Kai Liu, Xuejin Zhang, and Jianan Huang*, Nanoscale 2026, in press, https://doi.org/10.1039/D5NR04125B
Tracking single-molecule ferritin reassembly and disassembly using polymer-coated nanopores, Mahya Assadipapari, Alireza Soleimania, Max Adam, Jiali Li, Arman Yousefi, Saaman Zargarbashi, Lei Xu, Max F. K. Wills, Hesna Kara, Marina Santana Vega, Alasdair W. Clark, Andrew J. Hudson, Jian-An Huang, Mohsen Rahmani, and Cuifeng Ying, Nanoscale 2026,18, 1045-1054. https://doi.org/10.1039/D5NR02885J

2025

Single-Molecule SERS Detection of Phosphorylation in Serine and Tyrosine Using Deep Learning-Assisted Plasmonic Nanopore, Yaltaye, Mulusew W, Yingqi Zhao, Kuo Zhan, Eva Bozo, Pei-Lin Xin, Vahid Farrahi, Francesco De Angelis, and Jian-An Huang*, The Journal of Physical Chemistry Letters, 2025, 16(33): 8418-8426. https://pubs.acs.org/doi/full/10.1021/acs.jpclett.5c01753
Online Iterative Learning Enhanced Sim-to-Real Transfer for Efficient Manipulation of Deformable Objects, Zuyan Chen, Jian-An Huang, Juha Röning, Leopoldo Angrisani, and Shuai Li*. Machine Intelligence Research, 2025, 22(4): 696-712. https://link.springer.com/article/10.1007/s11633-025-1566-0
Single-Molecule Resolution of Oligopeptides in Anti-Aging Cosmetics Combined with Nanopore Readouts and Deep Learning Model, Daixin Liu, Xun Chen, Meili Ren, Yongxiang Ren, Mulusew W. Yaltaye, Jian-An Huang, Rong Tian, Liyuan Liang*, and Chunyu Zeng*. Chemical Engineering Journal, 2025, 519: 165442. https://doi.org/10.1016/j.cej.2025.165442
Single-Molecule SERS Discrimination of Proline from Hydroxyproline Assisted by a Deep Learning Model, Yingqi Zhao, Kuo Zhan, Pei-Lin Xin, Zuyan Chen, Shuai Li, Francesco De Angelis, and Jian-An Huang*. "Single-Molecule SERS Discrimination of Proline from Hydroxyproline Assisted by a Deep Learning Model." Nano Letters, 2025, 25(18): 7499-7506. DOI: 10.1021/acs.nanolett.5c01177
Surface-Enhanced Raman Spectroscopy for Biomedical Applications: Recent Advances and Future Challenges, Linley Li Lin, Ramon Alvarez-Puebla, Luis M. Liz-Marzán, Matt Trau, Jing Wang, Laura Fabris, Xiang Wang, Guokun Liu, Shuping Xu, Xiao Xia Han, Liangbao Yang, Aiguo Shen, Shikuan Yang, Yikai Xu, Chunchun Li, Jinqing Huang, Shao-Chuang Liu, Jian-An Huang, Indrajit Srivastava, Ming Li, Limei Tian, Lam Bang Thanh Nguyen, Xinyuan Bi, Dana Cialla-May, Pavel Matousek, Nicholas Stone, Randy P. Carney, Wei Ji, Wei Song, Zhou Chen, In Yee Phang, Malou Henriksen-Lacey, Haoran Chen, Zongyu Wu, Heng Guo, Hao Ma, Gennadii Ustinov, Siheng Luo, Sara Mosca, Benjamin Gardner, Yi-Tao Long, Juergen Popp, Bin Ren, Shuming Nie, Bing Zhao, Xing Yi Ling*, Jian Ye*. ACS applied materials & interfaces, 2025, 17(11): 16287-16379. DOI: 10.1021/acsami.4c17502

2024

Directly Characterizing the Capture Radius of Tethered Double-Stranded DNA by Single-Molecule Nanopipette Manipulation, Bohua Yin, Shaoxi Fang, Bin Wu, Wenhao Ma, Daming Zhou, Yajie Yin, Rong Tian, Shixuan He, Jian-An Huang, Wanyi Xie, Xing-Hua Zhang*, Zuobin Wang*, Deqiang Wang*, ACS Nano, 2024, https://pubs.acs.org/doi/abs/10.1021/acsnano.4c05605
Surface‐Enhanced Raman Scattering Imaging Assisted by Machine Learning Analysis: Unveiling Pesticide Molecule Permeation in Crop Tissues,Wang, Xiaotong, Xiaomeng Sun, Zhehan Liu, Yue Zhao, Guangrun Wu, Yunpeng Wang, Qian Li, Chunjuan Yang, Tao Ban, Yu Liu, Jian‐An Huang, Yang Li*, Advanced Science,2024,11(32). DOI:10.1002/advs.202405416
Unmasking Bacterial Identities: Exploiting Silver Nanoparticle 'Masks' for Enhanced Raman Scattering in the Rapid Discrimination of Diverse Bacterial Species and Antibiotic-Resistant Strains, Wang, Yunpeng, Shen Jiang, Qiang Fu, Xiaotong Wang, Zhe Zhang, Xiaoming Lyu, Xinyu Yao, Zhangyi Qu, Yingqi Zhao, Jian-An Huang, Yang Li*, Analytical chemistry, 2024, 96(21),8566-8575. DOI:10.1021/acs.analchem.4c00622
All-in-One Electrokinetic Strategy Coupled with a Miniaturized Chip for SERS Detection of Multipesticides, Lu Chen, Rui-Qi Peng, Wei Deng, Jian-An Huang*, Dan Li*, Analytical Chemistry,2024, 96(24):9834-9841. DOI:10.1021/acs.analchem.4c00179

2023

Label-free therapeutic drug monitoring in human serum by the 3-step surface enhanced Raman spectroscopy and multivariate analysis,Wang Yunpeng, Cuiwei Yu, Haoyu Ji, Zhehan Liu, Xiaotong Wang, Yi Ji, Xiaomeng Sun, Yue Zhao, Xiaohong X. Qiu, Ting Zhang, Jing Li, Xin Liu, Xin Lv, Benzhi Cai, Yingqi Zhao, Jian-An Huang, Yang Li*, Chemical Engineering Journal ,2022,452(4):139588. DOI:10.1016/j.cej.2022.139588
Plasmonic Bowl-Shaped Nanopore for Raman Detection of Single DNA Molecules in Flow-Through, Yingqi Zhao, Aliaksandr Hubarevich, Angela Federica De Fazio, Marzia Iarossi, Jian-An Huang*, Francesco De Angelis*, Nano Letters,2022,23(11),4830-4836.DOI:10.1021/acs.nanolett.3c00340
Hyperbolic metamaterial nanoparticles random array for thermoplasmonics in the II and III near-infrared windows, Zhao, Yingqi, Marzia Iarossi, N. Maccaferri, Lieselot Deleye, Giovanni Melle, Jian‐An Huang, Francesco Tantussi, Tommi Isoniemi and Francesco De Angelis*, Applied Physics Letters,2020,122(5),052201.DOI:10.1063/5.0132172
Band gap tuning of Sn1-xCexO2 nanoflower for improved SERS activity of bioassay, Lingling Jiang, Jiale Zhang, Hongying Li, Chaoqin He, Ruo Yuan, Yingqi Zhao, Jian-An Huang, Ying Zhuo, Xia Yang, Chemical Engineering Journal ,2023,461(19):142102. DOI:10.1016/j.cej.2023.142102
High‐Density Plasmonic Nanopores for DNA Sensing at Ultra‐Low Concentrations by Plasmon‐Enhanced Raman Spectroscopy, Marzia Iarossi, Daniel Darvill, Aliaksandr Hubarevich, Jian-An Huang, Yingqi Zhao, Angela Federica De Fazio, Devin B. O'Neill, Francesco Tantussi, Francesco De Angelis, Iarossi, Advanced Functional Materials,2023,33. DOI:10.1002/adfm.202301934
Detection of Cell-Derived Exosomes Via Surface-Enhanced Raman Scattering Using Aggregated Silver Nanoparticles, Yang Li, Yunpeng Wang, Jinwei Tian, Jian-An Huang*, Methods Mol Biol,2023,2668,15-22. DOI: 10.1007/978-1-0716-3203-1_2
Plasmonic nanopore array to detect translocating DNA and proteins at single molecule level by Raman Spectroscopy, Iarossi, Marzia, Daniel Darvill, Jian‐An Huang, Aliaksandr Hubarevich, Yingqi Zhao and F. De Angelis, Seventeenth International Congress on Artificial Materials for Novel Wave Phenomena (Metamaterials) ,2023, X-090-X-092. DOI:10.1109/Metamaterials58257.2023.10289343

2022

Microcontact printing of gold nanoparticle at three-phase interface as flexible substrate for SERS detection of MicroRNA, Hongying Li, Haina Zhang, Wei Luo, Ruo Yuan, Yingqi Zhao, Jian-An Huang *, Xia Yang *, Analytica Chimica Acta ,2022, 340380. DOI: 10.1016/j.aca.2022.340380
Gap controlled self-assembly Au@ Ag@ Au NPs for SERS assay of thiram, Jiale Zhang, Caijun Wu, Ruo Yuan, Jian-An Huang, Xia Yang*, Food Chemistry, 2022,390, 133164. DOI: 10.1016/j.foodchem.2022.133164
Probing ND7/23 Neuronal Cells Before and After Differentiation with SERS using Sharp-tipped Au Nanopyramid Arrays, Marzia Iarossi, Aliaksandr Hubarevich, Giuseppina Iachetta, Michele Dipalo, Jian-An Huang, Daniel Darvill*, Francesco De Angelis*, Sensors and Actuators B: Chemical ,2022, 131724. DOI: 10.1016/j.snb.2022.131724
Label-Free Optical Analysis of Biomolecules in Solid-State Nanopores: Toward Single-Molecule Protein Sequencing, Yingqi Zhao, Marzia Iarossi, Angela Federica De Fazio, Jian-An Huang*, and Francesco De Angelis*, ACS Photonics, 2022, 9 (3), 730-742. DOI: 10.1021/acsphotonics.1c01825
Surface-enhanced Raman spectroscopy detection of organic molecules and in situ monitoring of organic reactions by ion-induced silver nanoparticle clusters, Xiaoyue Li, Xiaotong Wang, Jiaxin Liu, Miaomiao Dai, Qianjun Zhang, Yang Li*, Jian-An Huang*, Physical Chemistry Chemical Physics ,2022, 24 (5),2826-2831, DOI: 10.1039/d1cp04857k

2021

Hyperbolic Nanoparticles on Substrate with Separate Optical Scattering and Absorption Resonances: A Dual Function Platform for SERS and Thermoplasmonics, Zhao, Yingqi*, Aliaksandr Hubarevich, Marzia Iarossi, Tetiana Borzda, Francesco Tantussi, Jian‐An Huang*, and Francesco De Angelis. Advanced Optical Materials (2021): 2100888. DOI: 10.1002/adom.202100888

Before joining University of Oulu

Breaking the symmetry of nanosphere lithography with anisotropic plasma etching induced by temperature gradients, D Darvill†, M Iarossi†, RMA Ekeroth, A Hubarevich, JA Huang, F De Angelis*, Nanoscale Advance, 3 (2), 359-369 (2021)
Multiplex characterization of single amino acid residue in polypeptide by single SERS hot spot, J.A. Huang*, M.V. Mousavi, Y.Q. Zhao, A. Hubarevich, G. Giovanini, W. Rocchia, D. Garoli*, and F. De Angelis, Angewandte Chemie International Edition, 59, 11423-11431 (2020), DOI: 10.1002/anie.202000489
λ-DNA Through porous materials – Surface Enhanced Raman Scattering in a simple plasmonic nanopore, A. Hubarevich†, J.A. Huang†, G. Giovanni†, A. Schirato, Y. Zhao, N. Maccaferri, F. De Angelis, A. Alabastri and D. Garoli*, Journal of Physical Chemistry C, 124 (41), 22663-22670 (2020)
A ring-shaped protein clusters gold nanoparticles acting as molecular scaffold for plasmonic surfaces, M. Ardini; J.A. Huang; V. Caprettini; F. De Angelis; F. Fata; I. Silvestri; A. Cimini; F. Giansanti; F. Angelucci; R. Ippoliti*, Biochimica et Biophysica Acta (BBA) - General Subjects, 1864 (2020) 129617. DOI: 10.1016/j.bbagen.2020.129617
SERS Discrimination of Single DNA Bases in Single Oligonucleotides by Electro-plasmonic Trapping, J.A. Huang, M.V. Mousavi, Y.Q. Zhao, A. Hubarevich, F. Omeis, G. Giovanini, D. Garoli*, and F. De Angelis*, Nature Communications 10, 5321 (2019) DOI:10.1038/s41467-019-13242-x
On-demand Intracellular Delivery of Single Particles in Single Cells by 3D Hollow Nanoelectrodes, J.A. Huang, V. Caprettini, Y.Q. Zhao, G. Melle, N. Maccaferri, M. Ardini, F. Tantussi, M. Dipalo and F. De Angelis*, Nano Letters, 19 (2), 722-731(2019)
Enhanced Raman investigation of cell membrane and intracellular compounds by 3D plasmonic nanoelectrode arrays, V. Caprettini, J.A. Huang, C. A. Gonano, F. Moia, M. Dipalo and F. De Angelis*, Advanced Science 5 (12), 1800560 (2018)
Low-dimensional Plasmonic Photodetectors: Recent Progress and Future Opportunities, J.A. Huang, L.B.Luo, Advanced Optical Materials, 6 (8), 1701282 (2018)
Live Intracellular Biorthogonal Imaging by Surface Enhanced Raman Spectroscopy using Alkyne-Silver Nanoparticles Clusters, M. Ardini, J.A. Huang, C. Sanchez, V. Caprettini, N. Maccaferri, G. Melle, G. Bruno, D. Garoli, F. De Angelis, Scientific Reports, 8, 12652 (2018)
Nanoporous Gold decorated with silver nanoparticles as large area efficient SERS substrate, M. Ardini, J.A. Huang, C. Sanchez, P. Ponzellini, N. Maccaferri, A. Jacassi, S. Cattarin, E. Calandrini, and D. Garoli*, Proceedings Volume 10346, Plasmonics: Design, Materials, Fabrication, Characterization, and Applications XV; 103460E (2017) doi: 10.1117/12.2273947
Soft electroporation for delivering molecules into tightly adherent mammalian cells through 3D hollow nanoelectrodes,V. Caprettini, A. Cerea, G. Melle, L. Lovato, R. Capozza, J.A. Huang, F. Tantussi, M. Dipalo and F. De Angelis*, Scientific Reports, 7, 8524 (2017)
Averaging effect on improving signal reproducibility of gap-based and gap-free SERS substrates based on ordered Si nanowire arrays, JA Huang†, Y Zhao†, X Zhu, W Zhang, RSC Advances, 7 (9), 5297-5305 (2017)
Superhydrophobic SERS chip based on a Ag coated natural taro-leaf, JA Huang, YL Zhang, Y Zhao, XL Zhang, ML Sun, WJ Zhang, Nanoscale, 8 (22), 11487-11493 (2016)
Monolithic Broadband InGaN Light-Emitting Diode, C Feng, JA Huang, HW Choi, ACS Photonics, 3 (7), 1294-1300 (2016)
Influence of strain on emission from GaN-on-Si microdisks, Y Zhang, JA Huang, KH Li, D Bai, Y Wang, T Wang, HW Choi, Journal of Physics D: Applied Physics, 49 (37), 375103 (2016)
Polarization-independent broadband plasmonic absorber based on a silicon-nanowire array decorated by gold nanoparticles at the optical regime, Y Han, JA Huang, XY Liu, XJ Zhang, JX Shi, CC Yan, Optics Express, 24 (9), 9178-9186 (2016)
SERS-enabled Lab-on-a-Chip Systems, JA Huang, YL Zhang*, H Ding, HB Sun*, Advanced Optical Materials, 3 (5), 618-633 (2015)
Highly Reproducible SERS Substrate based on Polarization-free Ag Nanoparticles Decorated SiO2/Si Core-shell Nanowires Array, XY Liu†, JA Huang†, B Yang, XJ Zhang* and YY Zhu*, AIP Advances 5, 057159 (2015)
Plasmonic Nanopillar Array Embedded Microfluidic Chips: an in situ SERS Monitoring Platform, YQ Zhao, YL Zhang, JA Huang, ZY Zhang, XF Chen* and WJ Zhang*, Journal of Materials Chemistry A, 3, 6408-6413 (2015)
Quantitative Analysis of Multiplex-components and Double Stranded DNA by Wide-range Surface-enhanced Raman Spectroscopy based on Ordered Ag/Si Nanowire Arrays, YQ Zhao, JA Huang, ZY Zhang, XF Chen* and WJ Zhang*, Journal of Materials Chemistry A, 2, 10218-10224 (2014)
Near-Infrared Light Photovoltaic Detector Based on GaAs Nanocone Array/Monolayer Graphene Schottky Junction, LB Luo*, JJ Chen, MZ Wang, H Hu, CY Wu, Q Li, L Wang, JA Huang*, FX Liang*, Advanced Functional Materials, 24 (19), 2794-2800, (2014)
The Effect of Plasmonic Nanoparticles on the Optoelectronic Characteristics of CdTe Nanowires, LB Luo*, XL Huang, MZ Wang, C Xie, CY Wu, JG Hu, L Wang, JA Huang*, Small, 10 (13), 2645-2652, (2014)
Ordered Ag/Si Nanowires Array: Wide-Range Surface-Enhanced Raman Spectroscopy for Reproducible Biomolecule Detection, JA Huang, YQ Zhao, XJ Zhang, LF He, TL Wong, YS Chui, WJ Zhang* and ST Lee*, Nano Letters, 13 (11), 5039–5045 (2013)
Silver Nanosheet-coated Inverse Opal Film as a Highly Active and Uniform SERS Substrate, LF He, JA Huang, TT Xu, LM Chen, K Zhang, ST Han, Y He* and ST Lee*, Journal of Materials Chemistry, 22, 1370 (2012)
Ultrafast Exciton Dynamics in Silicon Nanowires, DA Wheeler, JA Huang, RJ Newhouse, WF Zhang, ST Lee,*and JZ Zhang*, Journal of Physical Chemistry Letters, 3, 766−771 (2012)
Enhanced Raman scattering from vertical silicon nanowires array, JA Huang, YQ Zhao, XJ Zhang, LB Luo, YK Liu, JA Zapien, C Surya, and ST Lee*, Applied Physics Letters 98, 183108 (2011)
Ordered Silicon Nanocones Arrays for Label-free DNA Quantitative Analysis by Surface-enhanced Raman Spectroscopy, TT Xu, JA Huang, LF He, Y He*, S Su and ST Lee*, Applied Physics Letters 99, 153116 (2011)
Surface-Enhanced Emission from Single Semiconductor Nanoribbons, XJ Zhang, H Tang, JA Huang, LB Luo, JA Zapien, and ST Lee*, Nano Letters, 11(11), 4626-4630 (2011)
Nitrogen deep accepters in ZnO Nanowires Induced by Ammonia Plasma, R Huang, SG Xu, WH Guo, L Wang, J Song, TW Ng, JA Huang, ST Lee, SW Du, and N Wang*, Applied Physics Letters 99, 143112 (2011)

Conference Contributions

Constructing single hot spot in liquid for surface enhanced Raman spectroscopic single-molecule sequencing (online oral presentation), Jian-An Huang, 第四届拉曼光谱网络会议（iCRS2022）, Shanghai Normal University, China, 22-23 September 2022.
Plasmonic hot spot engineering for reliable SERS biosensors: from reproducible detection, quantitative analysis to single-molecule sequencing (online oral presentation), Jian-An Huang, Metanano school of optical biosensing (online), ITMO University, Russia, 15-19 August 2022.
Plasmonic Nanogap-enhanced Single-molecule Raman Spectroscopy: Towards Single-protein Raman Sequencing (online oral presentation), Y Zhao, M Iarossi, A F De Fazio, J.A. Huang*, F De Angelis, 12th International Conference on Metamaterials, Photonic Crystals and Plasmonics (META 2022), Torremolinos, Spain, 19 – 22 July, 2022.
Plasmonic Nanogap-enhanced Single-molecule Raman Spectroscopy: Towards Single-protein Raman Sequencing (oral presentation), Y Zhao, M Iarossi, A F De Fazio, J.A. Huang*, F De Angelis, NanoPlasm 2022, Cetraro, Italy, 13-17 June 2022.
Digital Health Management of Chronic Diseases at Aged Population by Point-of-care Diagnostic Devices and Big-data platform (Poster presentation), Peng Wan*, Ting Hou, Jian-An Huang*, The 26th Finnish National Conference on Telemedicine and eHealth, "eHealth in a Lifecycle", Oulu, Finland, 7th – 8th October 2021.
Single-molecule detection of DNA and proteins in a plasmonic nanopore by Surface Enhanced Raman Spectroscopy (SERS) (Invited oral presentation), J.A. Huang, 31st Anniversary World Congress on Biosensors (BIOSENSORS 2021), Online, 26 - 29 July 2021

Before joining University of Oulu

J.A. Huang, M.V. Mousavi, Y.Q. Zhao, A. Hubarevich, F. Omeis, G. Giovanini, D. Garoli, and F. De Angelis*, Single-Molecule SERS Detection of DNA Bases in oligonucleotides by Controllable Electro-plasmonic Trapping of Single Nanoparticles in Plasmonic Nanoholes (Oral presentation online), Single-Molecule Sensors and NanoSystems International Conference (S3IC), Barcelona, Spain, November 9 - 11, 2020
Huang J.-A., Caprettini V., Zhao Y.Q., Melle G., Deleye L., Maccaferri N., Zambrana-Puyalto X., Tantussi F., Dipalo M., De Angelis F.*, Controlled Intracellular Delivery of Single Particles in Single Cells by Plasmonic Hollow Nanoelectrodes (Oral presentation), Nanophotonics and Micro/Nano Optics International Conference (NANOP) 2019, 4 – 6 September, 2019, Munich, Germany
Zhao Y., Maccaferri N., Iarossi M., Isoniemi T., Huang J.A., Parracino A., Strangi G., De Angelis F.*, Hyperbolic Metamaterial Nanostructures for Arbitrary Control of Scattering and Absorption of Light (Oral presentation), Nanophotonics and Micro/Nano Optics International Conference (NANOP) 2019, 4 – 6 September, 2019, Munich, Germany
Garoli D., Huang J.A., De Angelis F.*, Single-molecule protein sequencing by means of hybrid nanopores - a new concept (Oral presentation), PROSEQO Workshop: New approaches for single protein sequencing , University of Barcelona, Barcelona, 21-22 Feb. 2019
J.A. Huang, V. Caprettini, Y.Q. Zhao, G. Melle, N. Maccaferri, M. Ardini, F. Tantussi, M. Dipalo and F. De Angelis*, Single-particle Intracellular Delivery by 3D Plasmonic Nanoelectrode (Oral presentation), 2018 International Conference on Nanoscience + Technology (ICN+T), 22 - 27 July 2018, Brno, Czech
J.A. Huang, V. Caprettini, C. A. Gonano, F. Moia, M. Dipalo and F. De Angelis*, Investigation of Cell Membrane Dynamics by 3D Nanoplasmonic Electrodes (Poster), 5th International workshop on Plasmonics July 5-7th, 2017, Lecce, Italy
M. Ardini, J.A. Huang, C. Sanchez, D. Garoli, N. Maccaferri, V. Caprettini, G. Melle, and F. De Angelis*, Plasmonic silver-alkyne nanoclusters as putative intracellular SERS-active probes (Oral presentation), 5th International workshop on Plasmonics July 5-7th, 2017, Lecce, Italy
Jian-An Huang, Yiyun Zhang, Cong Feng, Hoi Wai Choi , Optical Investigation of Ion Damage and Strain Relaxation On Plasma-etched InGaN/GaN Microdisks by Scanning Near-field Optical Microscopy and Spectroscopy (Poster), 11th International Conference on Nitride Semiconductors (ICNS-11), August 30th - September 4th, 2015, Beijing, China.
Yiyun Zhang, Jian-An Huang, T. Wang, H. W. Choi, Strain Relaxation Effect in InGaN/GaN Microdisks Pivoted on Si (Oral presentation), 11th International Conference on Nitride Semiconductors (ICNS-11), August 30th - September 4th, 2015, Beijing, China.
Cong Feng, Jian-An Huang, Hoi Wai Choi, Tuning the Recombination Rate of InGaN/GaN nanopillars with Voltage-induced Surface Charge Regions (Poster), 11th International Conference on Nitride Semiconductors (ICNS-11), August 30th - September 4th, 2015, Beijing, China.
Y. F. Cheung, K. H. Li, J. A. Huang and H. W. Choi, Photo-Electro-Vibrational-Thermal Characterization of Epi-Flexible GaN Light-Emitting Diodes (Oral presentation), 11th International Conference on Nitride Semiconductors (ICNS-11), August 30th - September 4th, 2015, Beijing, China.
Jian-An Huang, Ying-Qi Zhao, Tai-Lun Wong, Ying-Seng Chiu, Xue-Jin Zhang, Shuit-Tong Lee, Ag/SiNWs Array for Uniform Multi-analyte Detection by Surface-Enhanced Raman Spectroscopy (Poster), E-MRS 2013 Spring Meeting, May 27 - 31, 2013, Strasbourg, France.
J A Huang and S T Lee, Surface-enhanced Raman Scattering (SERS) from Ag/SiNW Arrays (Oral presentation), CityU-USTC 6th PhD Student Workshop, 10 – 11 April, 2012, Hefei, China
Jian-An HUANG, Ying-Qi ZHAO, Xue-Jin ZHANG, Lin-Bao LUO, Charles SURYA, Shuit-Tong LEE*, Enhanced Raman scattering from ordered vertical silicon nanowires array (Poster), 2011 MRS Spring Meeting, 25 – 29 April, 2011, San Francisco, USA
J A Huang and S T Lee, Enhanced Raman Scattering from Vertical Silicon Nanowires Arrays (Oral presentation), CityU-USTC 5th PhD Student Workshop, 18 – 19 April, 2011, Suzhou, China
HUANG Jianan, ZHAO Yingqi, ZHANG Xuejin, LUO Linbao, Charles SURYA, LEE Shuit-Tong*, Enhanced Raman Scattering from Large-area Ordered Silicon Nanowires Array with Tunable Size (Poster), the 8th Cross-Strait Workshop on Nano Science & Technology, 19 – 22, December, 2010, Hong Kong SAR, China

Full publication list in Google Scholar

Research ID in Web of Science

International collaborations

Dr. Francesco De Angelis, Italian Institute of Technology (Genova, Italy).

Dr. Michael Chen, The University of Edinburgh (Edinburgh, UK).

Dr. Humeyra Caglayan, Tampere University (Tampere, Finland).

Dr. Cuifeng Ying, Nottingham Trent University (Nottingham, UK).

Prof. Linbao Luo, Hefei University of Technology (Hefei, China).

Prof. Xuejin Zhang, Nanjing University (Nanjing, China).

Prof. Xia Yang, Southwest University (Chongqing, China).

The Jianan Lab of Nanophotonic Biosensors

Research group information

Unit and faculty

Contact information

Research group leader

Researchers

Research group description

Projects

RamanProSeq: Plasmonic nanopore technologies for automatic manipulation and Raman spectroscopic sequencing of single proteins

Next generation molecular sensing (NGSens)

Digital Raman assay for single-molecule protein sequencing in single live cells (LiveProSeq)

A wearable Raman sensor for monitoring metabolic secretions in sweat

Label-free single-molecule digital Raman assay for quantification of low abundance biomarkers (DigiRaman)

Plasmonic two-nanopore technology for single-molecule protein sequencing (TwoPoreProSeq)

Wearable Lab-in-a-watch Optical Sensor for Non-invasive Sweat Diagnosis

Research

Openning

News

Alumni

Publication

Full publication list in Google Scholar

Research ID in Web of Science

International collaborations

Teaching

Laser safety online training

080922S Optical Microscopy and Spectroscopic Imaging

744639S Biosensors and Introduction to Microfabrication

Chemical safety online training

New solutions for personalised medicine – University of Oulu receives major EU funding for protein sequencing technology

Pioneering technology for early disease detection under development at the University of Oulu