The Jianan Lab of Nanophotonic Biosensors

Because proteins cannot be amplified, current protein analysis and sequencing relies on mass spectroscopy that generally needs 1 billion copies of the proteins. The low sensitivity of mass spectroscopy leads to a huge challenge in detection of low-abundance proteins in human biofluids and thus spurs extensive research interests in single-molecule protein sequencing. Jian-an developed a plasmonic nanopore system by electro-plasmonic trapping of a gold nanoparticle in a gold nanopore to generate single SERS hot spot. The nanopore was so sensitive and localized that it not only distinguished single DNA bases in single single-strand DNA [1] but also discriminated single amino acid residue in single polypeptide [2]. In addition to the sub-molecular resolution, the plasmonic nanopre system has demonstrated single-molecule detection of 10 different types of amino acids by their Raman spectra [2], which has paved a completely new way to single-molecule protein sequencing. To apply the nanopore system to single-molecule sequencing, a microfluidic method will be integrated to stretch the DNA or protein molecules and slow down their passage through the nanopore.

Related works:

1. J.A. Huang et al., SERS discrimination of single DNA bases in single oligonucleotides by electro-plasmonic trapping, Nature Communications 10, 5321 (2019)

2. J.A. Huang*et al., Multiplex characterization of single amino acid residue in polypeptide by single SERS hot spot, Angewandte Chemie International Edition, 59, 11423-11431 (2020)

Wearable non-invasive molecular sensing technology will be an essential part for future personalized medicine. Current non-invasive wearable devices monitor heart rate and other physical, kinetic values such as temperature and body gestures, steps and location. It would be of tremendous added value if they could identify and quantify health factors that take part in control of human physiology, such as sweat glucose molecules that reflects blood glucose level. Failure to control blood glucose increases Body Mass Index, resulting in tremendous health risks in current society. This project aims to develop a wearable optical sensor on skin by integrating an electro-plasmonic chip [1] into an optical watch that together allows controllable sweat stimulation and on-watch continuous detection of the sweat glucose. When fully implemented, we plan to explore integration of an spectrometer into the watch and extend the lab-in-a-watch sensor to detect extracellular vesicles in sweat.

Reference:

He, Lifang, Jianan Huang, Tingting Xu, Limiao Chen, Kui Zhang, Suting Han, Yao He, and Shuit Tong Lee. "Silver nanosheet-coated inverse opal film as a highly active and uniform SERS substrate." Journal of Materials Chemistry 22, no. 4 (2012): 1370-1374.

Single-cell analysis provides important information of biology that are masked in analysis of cell populations and tissues. Current single-cell analysis techniques allow cell analysis at the genomic, transcriptomic and proteomic levels but have to lyse the cells to extract the cytosolic contents. Thus, it hampers real-time monitoring of intracellular dynamic transformations, such as differentiation and gene expression of single living cells, and, more importantly, interconnection between living cells with time. What is worse, the amount of extracted molecules from single cell were too little to be analyzed by conventional methods such as mass spectrometery.

The research goal of this topic is to develop optical biosensing methods using surface-enhanced Raman spectroscopy (SERS) as the sensing function for single-molecule and real-time single-cell analysis. A hollow nanoelectrode system is used to electroporate cell membrane, after which molecules or nanoparticles can be delivered into cytosol.[1] Alternatively, It can extract and sample intracellular content from specific cell into the microfluidic channel by electrical bias.[2] The extract can be detected by the plasmonic nanopores for single-molecule SERS characterization of the intracellular content.[3] By cycling sampling and detection, the integrated system will find broad applications for different cellular systems, such as cancer cells response to drugs.

Related works:

1. J.A. Huang et al., On-Demand Intracellular Delivery of Single Particles in Single Cells by 3D Hollow Nanoelectrodes, Nano letters 19 (2), 722–731 (2019)
2. Caprettini, Valeria, et al. "Enhanced Raman investigation of cell membrane and intracellular compounds by 3D plasmonic nanoelectrode arrays." Advanced Science 5.12 (2018): 1800560.
3. Huang, Jian‐An, et al. "Multiplexed discrimination of single amino acid residues in polypeptides in a single SERS hot spot." Angewandte Chemie International Edition 59.28 (2020): 11423-11431.

• 13.12.2024: #Grant# Congratulations for Caglar, Jianan, Simo, Aki, and Seppo together to receive the 3-year consortium Next Generation Molecular Sensing project from Jane and Aatos Erkko Foundation.
• 18.11.2024: #Welcome# Prof. Yang Li join our group as a Project Researcher for single-molecule sequencing.
• 21.-30.10.2024: #Visit# Mr. Zhiguo Zhu, a PhD student from Prof. Linbao Luo's group from Hefei University of Technology visited our group for the research of micro-controller and FPGA for single molecule sequencing.
• 17.-18.10.2024: #Conference# Jianan, Jane and Aki hold the Cancer Metabolism and Nutrition Symposium at University of Oulu, and welcome Prof. Xia Yang and Prof. Yang Li attend the symposium.
• 01.09.2024: #Welcome# Welcome Ms. Ling Liu joints our group as an PhD student supported by the PhD scholarship from the China Scholarship Council.
• 01.09.2024: #Celebrate# Congratulate Dr. Kuo Zhan to start his 3-year Data4Healthcare postdoctoral position to develop deep-learning algorithms for single-molecule Raman sequencing.
• 08.-10.08.2024: #Visit# Prof. Linbao Luo from Hefei University of Technology visited our group and discussed with Mulusew on AI data analysis.
• 28.07.-02.08.2024: #Conference# Jianan with Eva and Mulusew joint the 28th International Conference on Raman Spectroscopy – ICORS 2024 at Rome and gave an invited presentation: "Plasmonic nanopores for single-molecule Raman sequencing".
• 16.-24.07.2024: #Visit# Prof. Wei-Hung Chiang from National Taiwan University of Science and Technology visited our group and give a seminar talk of "Microplasma Engineering of Nanomaterials Synthesis and Applications".
• 25.06.2024: #Grant# Congratulations for Jianan, Dr. Yang Li and Prof. Shuai Li to get the PoC funding 2024: "Wearable biochips for on-demand sweat stimulation and continuous sensing".
• 15.06.-13.07.2024: #Visit# During a 4-week China trip, Jianan visited Foshan University, Chongqing Institute of Green and Intelligent Technology, Southwest University, Institute of Modern Physics (Lanzhou), Xián Jiaotong University, Southeast University, Nanjing University, Hangzhou Normal University, Zhejiang Lab, University of Science and Technology of China, Anhui Medical University and Hefei University of Technology.
• 04.04.2024: #Visit# Dr. Francesco De Angelis from Italian Institute of Technology visited our group and give a talk of "Hybrid interfaces between nanosensors and living cells" at the Biocenter Oulu seminar.
• 26.02.2024: #Grant# Jianan is granted the Research Council of Finland and the National Science and Technology Council (NSTC) mobile funding with Taiwan Prof. Wei-Hung Chiang on the project: Plasmonic rotation of single plasma-engineered Janus nanoparticle in nanopores for single-molecule Raman sequencing.
• 06.02.2024: #Conference# Dr. Kuo Zhan gave a oral presentation of "Label-free detection of exosomes from different cellular sources based on surface-enhanced Raman spectroscopy combined with machine learning" at the FISEV 2024 Annual Meeting at University of Oulu.
• 05.02.2024: #Visit# Dr. Yingqi Zhao was invited to visit Prof. Jinglai Duan's group at Materials Research Center, Institute of Modern Physics, Chinese Academy of Science, Lanzhou, China to give a seminar talk: "Plasmonic Nanopore Surface-enhanced Raman Spectroscopic (SERS) Sensors for Single DNA/Protein analysis" and promote collaboration.
• 04.01.2024: #Welcome# Welcome Iuliia Sheinman to join our group as a PhD student in the Academy Research Fellow project of Plasmonic two-nanopore technology for single-molecule protein sequencing (TwoPoreProSeq).
• 02.01.2024: #Celebrate# Congratulate Ms. Peilin Xin and Mr. Yuge Liang for being promoted as PhD students in the BCO project of Digital Raman assay for single-molecule protein sequencing in single live cells (LiveProSeq).

• 11.12.2023: #Welcome# Welcome Dr. Kuo Zhan to join our group as a postdoctoral researcher for wearable optical biosensors of sweat!
• 01.11.2023: #Welcome# Welcome Ms. Peilin Xin to join our group as a research assistant for single-molecule Raman sequencing!
• 09.10.2023: #Welcome# Welcome Mr. Alireza Soleimanian and Mr. Yuge Liang to join our group as the I4World doctoral student and research assistant!
• 04.09.2023: #Grant# Jian-An is granted the Biocenter Oulu project (2024-2027): Digital Raman assay for single-molecule protein sequencing in single live cells (LiveProSeq)
• 01.09.2023: #Celebrate# Congratulate Ms. Yuan Zhang for being promoted as a PhD student in single-molecule SERS biosensors in our group.
• 24.08.2023: #Welcome# Welcome Prof. Linbao Luo from Hefei University of Technology, China to visit our group during 24-28.8.2023.
• 03.07.2023: #Welcome# Welcome Mr. Radoslaw Plawecki to join our group as a trainee!
• 01.06.2023: #Publication# Congratulate Yingqi's work of plasmonic bowl-shaped nanopore for single-molecule SERS published by Nano Letters.
• 30.05.2023: #Scholarship# Congratulate Ms. Yuan Zhang for receiving 4-year China Scholarship Council (CSC) PhD scholarship under Jian-An's host.
• 12.04.2023: #Grant# Jian-An is granted the DigiHealth NRI funding: A wearable Raman sensor for monitoring metabolic secretions in sweat.
• 27.03.2023: #Welcome# Welcome Ms. Yuan Zhang joins our group as a research assistant!
• 21.02.2023: #Grant# Jian-An is granted the Funding for mobility cooperation with China for joint research of automatic control of single-protein unfolding and motion in nanopores for Raman sequencing with Prof. Linbao Luo in Hefei University of Technology, China.
• 20.01.2023: #Celebrate# Congratulate Mulusew Wondie Yaltaye promoted as a PhD student in the project of Label-free digital Raman assay for quantification of low-abundance biomarkers (DigiRaman) in our group.

• 01.12.2022: #Welcome# Welcome Dr. Yang Li to join our group as a visiting research scientist!
• 21.11.2022: #Welcome# Welcome Xiang An to join our group as a visiting MSc student!
• 07.11.2022: #Welcome# Welcome Miaomiao Li to join our group as a CSC-funded PhD student!
• 11.10.2022: #Welcome# Welcome Mulusew Wondie Yaltaye to join our group as a Research Assistant!
• 03.10.2022: #Welcome# Welcome Éva Bozó to join our group as a PhD student in the Academy Research Fellow project (TwoPoreProSeq) for single-molecule protein sequencing.
• 14.09.2022: #Grant# Jian-An is granted the Emerging Project: Label-free digital Raman assay for quantification of low-abundance biomarkers (DigiRaman)
• 09.09.2022: #Celebrate# Congratulations to the team including Éva Bozó and Xiuli Wang from my group in winning the "Wildest Idea" award in the Innofest 2022 competition by proposing a portable nanopore Raman biosensors for diabetes diagnosis.
• 01.09.2022: #Welcome# Welcome Dr. Esa-Ville Immonen join our group as a postdoc fellow!
• 12.05.2022: #Grant# Jian-An is granted the Academy Research Fellow funding (2022-2027) for single-molecule protein sequencing.
• 01.08.2022: #Welcome# Welcome Joonas Kujala join our group as a research assistant!
• 20.06.2022: #Grant# Jian-An, Yingqi and Simo Saarakkala are granted the PoC funding: Surface-enhanced Raman spectroscopy (SERS) based rheumatoid arthritis diagnosis.
• 30.05.2022: #Welcome# Welcome Zakariya Ait Athmane to join our group as a trainee!
• 08.04.2022: #Welcome# Welcome Dr. Xiuli Wang to join our group as a visiting professor!
• 01.01.2022: #Welcome# Welcome Dr. Yingqi Zhao to join our group as a senor research fellow!

• 11.10.2021: #Celebrate# Jian-an is selected as a member in the Nano Letters Early Career Board.
• 30.08.2021: #Grant# Jian-an got the Finland-China Food and Health (FCFH) Network grant (28 000 EUR) with Prof. Tapio Fabritius to fund a visit of Chinese researcher for joint research of Nanophotonic biosensors for label-free analysis of biomarkers in biological fluids.
• 20.08.2021: #Grant# Jian-an got an Tandem Industry Academica (TIA) 2021 grant to develop an wearable lab-on-a-watch optical sensor for sweat diagnosis.

• Mr. Radosław Pławecki, Wroclaw University of Science and Technology (Poland): internship in July - September 2023.
• Mr. Xiang AN, visiting MSc student from Hefei University of Technology (China) during November 2022 - March 2023.
• Dr. Xiuli Wang, Ph.D., China Scholarship Council-funded visiting scholar from Hangzhou Normal University (China) during April 2022 - March 2023.
• Mr. Joonas Kujala, University of Oulu (Finland): Research Assistant in June 2022 - January 2023.
• Mr. Zakariya Ait Athmane, University of Strasbourg (France): internship in May - August 2022.

2023

1. Yingqi Zhao, Aliaksandr Hubarevich, Angela De Fazio, Marzia Iarossi, Jian-An Huang*, and Francesco De Angelis*, Plasmonic bowl-shaped nanopore for Raman detection of single DNA molecules in flow-through, Nano Letters, 23, 11, 4830–4836 (2023).
2. L Jiang, J Zhang, H Li, C He, R Yuan, Y Zhao, JA Huang, Y Zhuo, X Yang*, Band gap tuning of Sn1-xCexO2 nanoflower for improved SERS activity of bioassay, Chemical Engineering Journal 461, 142102 (2023)
3. Yingqi Zhao, Marzia Iarossi, Nicolò Maccaferri, Lieselot Deleye, Giovanni Melle, Jian-An Huang, Giuseppina Iachetta, Marta D'Amora, Francesco Tantussi, Tommi Isoniemi, and Francesco De Angelis*, Hyperbolic Metamaterial Nanoparticles Random Array for Thermoplamonics in the II and III Near-Infrared Windows, Applied Physics Letters 122, 052201 (2023)
4. Yunpeng Wang, Cuiwei Yu, Haoyu Ji, Zhehan Liu, Xiaotong Wang, Yinghe Ji, Xiaomeng Sun, Yue Zhao, Xiaohong Qiu, Ting Zhang, Jing Li, Xin Liu, Xinpeng Lv, Benzhi Cai, Yingqi Zhao, Jian-An Huang, Yang Li*, Label-free therapeutic drug monitoring in human serum by the 3-step surface enhanced Raman spectroscopy and multivariate analysis, Chemical Engineering Journal 452, 139588 (2023)

2022

1. Hongying Li, Haina Zhang, Wei Luo, Ruo Yuan, Yingqi Zhao, Jian-An Huang*, Xia Yang*, Microcontact printing of gold nanoparticle at three-phase interface as flexible substrate for SERS detection of MicroRNA, Analytica Chimica Acta 1229, 340380 (2022)
2. Marzia Iarossi, Aliaksandr Hubarevich, Giuseppina Iachetta, Michele Dipalo, Jian-An Huang, Daniel Darvill*, Francesco De Angelis*, Probing ND7/23 Neuronal Cells Before and After Differentiation with SERS using Sharp-tipped Au Nanopyramid Arrays, Sensors and Actuators B: Chemical (2022): 131724.
3. Jiale Zhang, Caijun Wu, Ruo Yuan, Jian-An Huang, Xia Yang*, Gap controlled self-assembly Au@ Ag@ Au NPs for SERS assay of thiram, Food Chemistry 390, 133164 (2022)
4. Yingqi Zhao, Marzia Iarossi, Angela Federica De Fazio, Jian-An Huang*, and Francesco De Angelis*, Label-Free Optical Analysis of Biomolecules in Solid-State Nanopores: Toward Single-Molecule Protein Sequencing, ACS Photonics, 2022 9 (3), 730-742.
5. Xiaoyue Li, Xiaotong Wang, Jiaxin Liu, Miaomiao Dai, Qianjun Zhang, Yang Li*, Jian-An Huang*, Surface-enhanced Raman spectroscopy detection of organic molecules and in situ monitoring of organic reactions by ion-induced silver nanoparticle clusters, Physical Chemistry Chemical Physics , 2022, 24 (5), 2826-2831.

2021

1. Zhao, Yingqi*, Aliaksandr Hubarevich, Marzia Iarossi, Tetiana Borzda, Francesco Tantussi, Jian‐An Huang*, and Francesco De Angelis, Hyperbolic Nanoparticles on Substrate with Separate Optical Scattering and Absorption Resonances: A Dual Function Platform for SERS and Thermoplasmonics, Advanced Optical Materials (2021): 2100888.

​Before joining University of Oulu

1. Breaking the symmetry of nanosphere lithography with anisotropic plasma etching induced by temperature gradients, ​D Darvill†, M Iarossi†, RMA Ekeroth, A Hubarevich, JA Huang, F De Angelis*, Nanoscale Advance, 3 (2), 359-369 (2021)
2. Multiplex characterization of single amino acid residue in polypeptide by single SERS hot spot, J.A. Huang*, M.V. Mousavi, Y.Q. Zhao, A. Hubarevich, G. Giovanini, W. Rocchia, D. Garoli*, and F. De Angelis, Angewandte Chemie International Edition, 59, 11423-11431 (2020), DOI: 10.1002/anie.202000489
3. λ-DNA Through porous materials – Surface Enhanced Raman Scattering in a simple plasmonic nanopore, A. Hubarevich†, J.A. Huang†, G. Giovanni†, A. Schirato, Y. Zhao, N. Maccaferri, F. De Angelis, A. Alabastri and D. Garoli*, Journal of Physical Chemistry C, 124 (41), 22663-22670 (2020)
4. A ring-shaped protein clusters gold nanoparticles acting as molecular scaffold for plasmonic surfaces, M. Ardini; J.A. Huang; V. Caprettini; F. De Angelis; F. Fata; I. Silvestri; A. Cimini; F. Giansanti; F. Angelucci; R. Ippoliti*, Biochimica et Biophysica Acta (BBA) - General Subjects, 1864 (2020) 129617. DOI: 10.1016/j.bbagen.2020.129617
5. SERS Discrimination of Single DNA Bases in Single Oligonucleotides by Electro-plasmonic Trapping, J.A. Huang, M.V. Mousavi, Y.Q. Zhao, A. Hubarevich, F. Omeis, G. Giovanini, D. Garoli*, and F. De Angelis*, Nature Communications 10, 5321 (2019) DOI:10.1038/s41467-019-13242-x
6. On-demand Intracellular Delivery of Single Particles in Single Cells by 3D Hollow Nanoelectrodes, J.A. Huang, V. Caprettini, Y.Q. Zhao, G. Melle, N. Maccaferri, M. Ardini, F. Tantussi, M. Dipalo and F. De Angelis*, Nano Letters, 19 (2), 722-731(2019)
7. Enhanced Raman investigation of cell membrane and intracellular compounds by 3D plasmonic nanoelectrode arrays, V. Caprettini, J.A. Huang, C. A. Gonano, F. Moia, M. Dipalo and F. De Angelis*, Advanced Science 5 (12), 1800560 (2018)
8. Low-dimensional Plasmonic Photodetectors: Recent Progress and Future Opportunities, J.A. Huang, L.B.Luo, Advanced Optical Materials, 6 (8), 1701282 (2018)
9. Live Intracellular Biorthogonal Imaging by Surface Enhanced Raman Spectroscopy using Alkyne-Silver Nanoparticles Clusters, M. Ardini, J.A. Huang, C. Sanchez, V. Caprettini, N. Maccaferri, G. Melle, G. Bruno, D. Garoli, F. De Angelis, Scientific Reports, 8, 12652 (2018)
10. Nanoporous Gold decorated with silver nanoparticles as large area efficient SERS substrate, M. Ardini, J.A. Huang, C. Sanchez, P. Ponzellini, N. Maccaferri, A. Jacassi, S. Cattarin, E. Calandrini, and D. Garoli*, Proceedings Volume 10346, Plasmonics: Design, Materials, Fabrication, Characterization, and Applications XV; 103460E (2017) doi: 10.1117/12.2273947
11. Soft electroporation for delivering molecules into tightly adherent mammalian cells through 3D hollow nanoelectrodes,V. Caprettini, A. Cerea, G. Melle, L. Lovato, R. Capozza, J.A. Huang, F. Tantussi, M. Dipalo and F. De Angelis*, Scientific Reports, 7, 8524 (2017)
12. Averaging effect on improving signal reproducibility of gap-based and gap-free SERS substrates based on ordered Si nanowire arrays, JA Huang†, Y Zhao†, X Zhu, W Zhang, RSC Advances, 7 (9), 5297-5305 (2017)
13. Superhydrophobic SERS chip based on a Ag coated natural taro-leaf, JA Huang, YL Zhang, Y Zhao, XL Zhang, ML Sun, WJ Zhang, Nanoscale, 8 (22), 11487-11493 (2016)
14. Monolithic Broadband InGaN Light-Emitting Diode, C Feng, JA Huang, HW Choi, ACS Photonics, 3 (7), 1294-1300 (2016)
15. Influence of strain on emission from GaN-on-Si microdisks, Y Zhang, JA Huang, KH Li, D Bai, Y Wang, T Wang, HW Choi, Journal of Physics D: Applied Physics, 49 (37), 375103 (2016)
16. Polarization-independent broadband plasmonic absorber based on a silicon-nanowire array decorated by gold nanoparticles at the optical regime, Y Han, JA Huang, XY Liu, XJ Zhang, JX Shi, CC Yan, Optics Express, 24 (9), 9178-9186 (2016)
17. SERS-enabled Lab-on-a-Chip Systems, JA Huang, YL Zhang*, H Ding, HB Sun*, Advanced Optical Materials, 3 (5), 618-633 (2015)
18. Highly Reproducible SERS Substrate based on Polarization-free Ag Nanoparticles Decorated SiO2/Si Core-shell Nanowires Array, XY Liu†, JA Huang†, B Yang, XJ Zhang* and YY Zhu*, AIP Advances 5, 057159 (2015)
19. Plasmonic Nanopillar Array Embedded Microfluidic Chips: an in situ SERS Monitoring Platform, YQ Zhao, YL Zhang, JA Huang, ZY Zhang, XF Chen* and WJ Zhang*, Journal of Materials Chemistry A, 3, 6408-6413 (2015)
20. Quantitative Analysis of Multiplex-components and Double Stranded DNA by Wide-range Surface-enhanced Raman Spectroscopy based on Ordered Ag/Si Nanowire Arrays, YQ Zhao, JA Huang, ZY Zhang, XF Chen* and WJ Zhang*, Journal of Materials Chemistry A, 2, 10218-10224 (2014)
21. Near-Infrared Light Photovoltaic Detector Based on GaAs Nanocone Array/Monolayer Graphene Schottky Junction, LB Luo*, JJ Chen, MZ Wang, H Hu, CY Wu, Q Li, L Wang, JA Huang*, FX Liang*, Advanced Functional Materials, 24 (19), 2794-2800, (2014)
22. The Effect of Plasmonic Nanoparticles on the Optoelectronic Characteristics of CdTe Nanowires, LB Luo*, XL Huang, MZ Wang, C Xie, CY Wu, JG Hu, L Wang, JA Huang*, Small, 10 (13), 2645-2652, (2014)
23. Ordered Ag/Si Nanowires Array: Wide-Range Surface-Enhanced Raman Spectroscopy for Reproducible Biomolecule Detection, JA Huang, YQ Zhao, XJ Zhang, LF He, TL Wong, YS Chui, WJ Zhang* and ST Lee*, Nano Letters, 13 (11), 5039–5045 (2013)
24. Silver Nanosheet-coated Inverse Opal Film as a Highly Active and Uniform SERS Substrate, LF He, JA Huang, TT Xu, LM Chen, K Zhang, ST Han, Y He* and ST Lee*, Journal of Materials Chemistry, 22, 1370 (2012)
25. Ultrafast Exciton Dynamics in Silicon Nanowires, DA Wheeler, JA Huang, RJ Newhouse, WF Zhang, ST Lee,*and JZ Zhang*, Journal of Physical Chemistry Letters, 3, 766−771 (2012)
26. Enhanced Raman scattering from vertical silicon nanowires array, JA Huang, YQ Zhao, XJ Zhang, LB Luo, YK Liu, JA Zapien, C Surya, and ST Lee*, Applied Physics Letters 98, 183108 (2011)
27. Ordered Silicon Nanocones Arrays for Label-free DNA Quantitative Analysis by Surface-enhanced Raman Spectroscopy, TT Xu, JA Huang, LF He, Y He*, S Su and ST Lee*, Applied Physics Letters 99, 153116 (2011)
28. Surface-Enhanced Emission from Single Semiconductor Nanoribbons, XJ Zhang, H Tang, JA Huang, LB Luo, JA Zapien, and ST Lee*, Nano Letters, 11(11), 4626-4630 (2011)
29. Nitrogen deep accepters in ZnO Nanowires Induced by Ammonia Plasma, R Huang, SG Xu, WH Guo, L Wang, J Song, TW Ng, JA Huang, ST Lee, SW Du, and N Wang*, Applied Physics Letters 99, 143112 (2011)

1. Constructing single hot spot in liquid for surface enhanced Raman spectroscopic single-molecule sequencing (online oral presentation), Jian-An Huang, 第四届拉曼光谱网络会议（iCRS2022）, Shanghai Normal University, China, 22-23 September 2022.
2. Plasmonic hot spot engineering for reliable SERS biosensors: from reproducible detection, quantitative analysis to single-molecule sequencing (online oral presentation), Jian-An Huang, Metanano school of optical biosensing (online), ITMO University, Russia, 15-19 August 2022.
3. Plasmonic Nanogap-enhanced Single-molecule Raman Spectroscopy: Towards Single-protein Raman Sequencing (online oral presentation), Y Zhao, M Iarossi, A F De Fazio, J.A. Huang*, F De Angelis, 12th International Conference on Metamaterials, Photonic Crystals and Plasmonics (META 2022), Torremolinos, Spain, 19 – 22 July, 2022.
4. Plasmonic Nanogap-enhanced Single-molecule Raman Spectroscopy: Towards Single-protein Raman Sequencing (oral presentation), Y Zhao, M Iarossi, A F De Fazio, J.A. Huang*, F De Angelis, NanoPlasm 2022, Cetraro, Italy, 13-17 June 2022.
5. Digital Health Management of Chronic Diseases at Aged Population by Point-of-care Diagnostic Devices and Big-data platform (Poster presentation), Peng Wan*, Ting Hou, Jian-An Huang*, The 26th Finnish National Conference on Telemedicine and eHealth, "eHealth in a Lifecycle", Oulu, Finland, 7th – 8th October 2021.
6. Single-molecule detection of DNA and proteins in a plasmonic nanopore by Surface Enhanced Raman Spectroscopy (SERS) (Invited oral presentation), J.A. Huang, 31st Anniversary World Congress on Biosensors (BIOSENSORS 2021), Online, 26 - 29 July 2021

Before joining University of Oulu

1. J.A. Huang, M.V. Mousavi, Y.Q. Zhao, A. Hubarevich, F. Omeis, G. Giovanini, D. Garoli, and F. De Angelis*, Single-Molecule SERS Detection of DNA Bases in oligonucleotides by Controllable Electro-plasmonic Trapping of Single Nanoparticles in Plasmonic Nanoholes (Oral presentation online), Single-Molecule Sensors and NanoSystems International Conference (S3IC), Barcelona, Spain, November 9 - 11, 2020
2. Huang J.-A., Caprettini V., Zhao Y.Q., Melle G., Deleye L., Maccaferri N., Zambrana-Puyalto X., Tantussi F., Dipalo M., De Angelis F.*, Controlled Intracellular Delivery of Single Particles in Single Cells by Plasmonic Hollow Nanoelectrodes (Oral presentation), Nanophotonics and Micro/Nano Optics International Conference (NANOP) 2019, 4 – 6 September, 2019, Munich, Germany
3. Zhao Y., Maccaferri N., Iarossi M., Isoniemi T., Huang J.A., Parracino A., Strangi G., De Angelis F.*, Hyperbolic Metamaterial Nanostructures for Arbitrary Control of Scattering and Absorption of Light (Oral presentation), Nanophotonics and Micro/Nano Optics International Conference (NANOP) 2019, 4 – 6 September, 2019, Munich, Germany
4. Garoli D., Huang J.A., De Angelis F.*, Single-molecule protein sequencing by means of hybrid nanopores - a new concept (Oral presentation), PROSEQO Workshop: New approaches for single protein sequencing , University of Barcelona, Barcelona, 21-22 Feb. 2019
5. J.A. Huang, V. Caprettini, Y.Q. Zhao, G. Melle, N. Maccaferri, M. Ardini, F. Tantussi, M. Dipalo and F. De Angelis*, Single-particle Intracellular Delivery by 3D Plasmonic Nanoelectrode (Oral presentation), 2018 International Conference on Nanoscience + Technology (ICN+T), 22 - 27 July 2018, Brno, Czech
6. J.A. Huang, V. Caprettini, C. A. Gonano, F. Moia, M. Dipalo and F. De Angelis*, Investigation of Cell Membrane Dynamics by 3D Nanoplasmonic Electrodes (Poster), 5th International workshop on Plasmonics July 5-7th, 2017, Lecce, Italy
7. M. Ardini, J.A. Huang, C. Sanchez, D. Garoli, N. Maccaferri, V. Caprettini, G. Melle, and F. De Angelis*, Plasmonic silver-alkyne nanoclusters as putative intracellular SERS-active probes (Oral presentation), 5th International workshop on Plasmonics July 5-7th, 2017, Lecce, Italy
8. Jian-An Huang, Yiyun Zhang, Cong Feng, Hoi Wai Choi , Optical Investigation of Ion Damage and Strain Relaxation On Plasma-etched InGaN/GaN Microdisks by Scanning Near-field Optical Microscopy and Spectroscopy (Poster), 11th International Conference on Nitride Semiconductors (ICNS-11), August 30th - September 4th, 2015, Beijing, China.
9. Yiyun Zhang, Jian-An Huang, T. Wang, H. W. Choi, Strain Relaxation Effect in InGaN/GaN Microdisks Pivoted on Si (Oral presentation), 11th International Conference on Nitride Semiconductors (ICNS-11), August 30th - September 4th, 2015, Beijing, China.
10. Cong Feng, Jian-An Huang, Hoi Wai Choi, Tuning the Recombination Rate of InGaN/GaN nanopillars with Voltage-induced Surface Charge Regions (Poster), 11th International Conference on Nitride Semiconductors (ICNS-11), August 30th - September 4th, 2015, Beijing, China.
11. Y. F. Cheung, K. H. Li, J. A. Huang and H. W. Choi, Photo-Electro-Vibrational-Thermal Characterization of Epi-Flexible GaN Light-Emitting Diodes (Oral presentation), 11th International Conference on Nitride Semiconductors (ICNS-11), August 30th - September 4th, 2015, Beijing, China.
12. Jian-An Huang, Ying-Qi Zhao, Tai-Lun Wong, Ying-Seng Chiu, Xue-Jin Zhang, Shuit-Tong Lee, Ag/SiNWs Array for Uniform Multi-analyte Detection by Surface-Enhanced Raman Spectroscopy (Poster), E-MRS 2013 Spring Meeting, May 27 - 31, 2013, Strasbourg, France.
13. J A Huang and S T Lee, Surface-enhanced Raman Scattering (SERS) from Ag/SiNW Arrays (Oral presentation), CityU-USTC 6th PhD Student Workshop, 10 – 11 April, 2012, Hefei, China
14. Jian-An HUANG, Ying-Qi ZHAO, Xue-Jin ZHANG, Lin-Bao LUO, Charles SURYA, Shuit-Tong LEE*, Enhanced Raman scattering from ordered vertical silicon nanowires array (Poster), 2011 MRS Spring Meeting, 25 – 29 April, 2011, San Francisco, USA
15. J A Huang and S T Lee, Enhanced Raman Scattering from Vertical Silicon Nanowires Arrays (Oral presentation), CityU-USTC 5th PhD Student Workshop, 18 – 19 April, 2011, Suzhou, China
16. HUANG Jianan, ZHAO Yingqi, ZHANG Xuejin, LUO Linbao, Charles SURYA, LEE Shuit-Tong*, Enhanced Raman Scattering from Large-area Ordered Silicon Nanowires Array with Tunable Size (Poster), the 8th Cross-Strait Workshop on Nano Science & Technology, 19 – 22, December, 2010, Hong Kong SAR, China