Virus Core Laboratory

The BCO Virus Core laboratory (contact persons) is a fully equipped Biosafety Level 2 cell-culture laboratory providing:

1) Services

CRISPR gene modification

  • CRISPR Feasibility (Feasibility of a cell line for CRISPR gene knockout modification by lentiviral transduction)
  • CRISPR Knockout (Generation of a CRISPR/Cas9-modified cell line)

Viral vectors

2) Laboratory facilities, training and scientific consultation

in virus technologies and an easy and cost-effective start for virus-based gene expression and gene-silencing studies.

All the necessary reagents, consumables and molecular tools are available for retroviral (MuLV), lentiviral and adenoviral gene transfer approaches. We also help in designing custom-made viral RNA interference (RNAi) gene-silencing constructs and CRISPR-based gene KO constructs and in planning genetic manipulation of mouse embryonic stem cells and/or generation of lenti-transgenic mouse models.

The Virus Core laboratory is accessible to trained users at any time. It contains two laminar hoods bookable via Asimov reservation system Laminar 1 and Laminar 2. A high-quality Zeiss inverted fluorescence microscope equipped with a sensitive camera and a standard stereomicroscope are available for imaging. Purification and concentration of viral vectors can be carried out by ultracentrifugation or FPLC chromatography. An Ultra-low-temperature freezer is provided for users to allow storage of viral preparations at the BCO Virus Core laboratory.


CRISPR Feasibility

  • Feasibility of a cell line for CRISPR gene knockout modification by lentiviral transduction
  • The service can be made for mycoplasma-tested cell lines or for primary cells
  • The cells are infected with GFP lentivirus and the resulting transduction efficiency is determined
  • The customer receives information if their target cell line is feasible for lentiCRISPR approach
  • The service price is 50 €
  • The service is delivered in 2-3 days after receiving the cells
  • Additional services: CRISPR Knockout

CRISPR Knockout

  • Preparation of a CRISPR gene modified cell line with lentivirus infection
  • The service requires CRISPR Feasibility information
  • The service includes design and cloning of gRNA into lentiviral CRISPR-vector, infection of target cells and selection of positively transduced cell population
  • The customer receives CRISPR knockout cell population
  • The service price is 3000 €
  • The service is delivered in 2 months after CRISPR Feasibility information

Lentivirus vector

  • Generation of recombinant lentiviral particles from lentiviral expression plasmid construct (provided by the customer)
  • Viral vectors can be concentrated by ultracentrifugation
  • The customer receives lentivirus vector (0.5 ml for concentrated prepps or 5-20 ml for unconcentrated prepps)
  • The service price is 500 €
  • The service is delivered within 2 weeks after receiving the plasmid
  • Additional services:
       • Purification of viral vectors by anion exchange HPLC, according to separate offer
       • Plasmid production 200 € (plasmid map available and only common enzymes needed)
       • Virus content by titration 110 €
  • Virus vector datasheet form
  • BCO Virus Core service order form for Retro/Lentiviruses

Retrovirus vector

  • Generation of recombinant murine retroviral particles from retroviral expression plasmid construct (provided by the customer)
  • Virus vector can be concentrated and purified using ultracentrifugation
  • The customer receives retroviral vector (0.5 ml for concentrated prepps or 5-20 ml for unconcentrated prepps)
  • The service price is 500 €
  • The service is delivered within 2 weeks after receiving of the plasmid
  • Additional services:
       • Purification of viral vectors by anion exchange HPLC,  according to separate offer
       • Plasmid production 200 € (plasmid map available and only common enzymes needed)
       • Virus content by titration 110 €
  • Virus vector datasheet form
  • BCO Virus Core service order form for Retro/Lentiviruses

Adenovirus vector

  • Transfection of a linearized adenoviral expression plasmid (provided by the customer) into adenoviral packaging system and generation of recombinant adenoviral vector
  • Viral vector is purified by Optiprep gradient isolation and viral content is determined by photometric measurement
  • The customer receives adenovirus vector
  • The service price is 500 €
  • The service is delivered within 2-3 weeks after delivery of the plasmid
  • Additional services:
       • Chromatographic purification available according to separate offer
       • Additional production of adenovirus vector from existing viral prepp 250 € (adeno-expansion)
       • Cloning service for adenoviral vector 500 €
  • Virus vector datasheet form
  • BCO Virus Core service order form for Adenoviruses

Contact persons:

Aki Manninen, Ph.D.,
Coordinator of the Virus Core laboratory
Tel. +358-(0)294 486081
E-mail: aki.manninen (at) oulu.fi
Room: 474B, Aapistie 5A

Jaana Träskelin
Technician
Tel. +358-(0)294 486065
E-mail: jaana.traskelin (at) oulu.fi
Room: 437A, Aapistie 5A

Last updated: 24.3.2020