Bio-Layer Interferometry

Introduction

Bio-Layer Interferometry (BLI) is another label-free technology that measures molecular interactions in real time for the purpose of detection, quantitation and kinetic analysis. Target molecules bind to the coated surface of the fiber-optic biosensors, forming a molecular layer that changes the spectral pattern of the reflected light. This technology is useful for basic research in protein-ligand interaction and for applications in biotherapeutic drug discovery and development. The facility is equipped with one advanced BLI instrument:

Octet Red 384 (ForteBio)

Octet Red 384 has measurement capacities ranging from 102-107 M-1s-1 for the association rate and 10-6-10-1 s-1 for the dissociation rate. The affinity constant KD ranges from 1mM to 10 pM. A number of specific biosensors are available from the manufacture.

Click here for Octet system details.

Sample requirements and sensor selection

Please discuss with the Core responsible person before the experiments.

Click here for more information.

Sample submission

Customers inside FBMM are suggested to use the in-house reservation system.
Customers from outside can contact the core responsible person by e-mail.

Prices

Academic service price: 35 EUR/hour.

For frequent user, academic self-service, or company service prices please contact Dr. Hongmin Tu.

Uses of sensors are not included in the price. The cost will be calculated separately according to the sensor purchasing prices.

Acknowledging the BCO core facility

The Oulu BCO core facilities are supported by funding from Biocenter Finland, Biocenter Oulu, the University of Oulu, FBMM, and regional funding.  We kindly ask the users to acknowledge this resource whenever publications are being finalised, for example:

"The use of the facilities  and expertise of the Biocenter Oulu biophysical protein analysis core facility, a member of Biocenter Finland, is gratefully acknowledged.“

In addition we ask the users to inform us whenever such publications have been accepted for publication.

Contact person:

Dr. Hongmin Tu
Faculty of Biochemistry and Molecular Medicine
University of Oulu
Aapistie 7B, 90014 Oulu, Finland
tel. +358-(0)294 485821
e-mail: hongmin.tu(at)oulu.fi

Last updated: 8.1.2019