Prof. Rikkert K. Wierenga, Ph.D.
Biocenter Oulu and Faculty of Biochemistry and Molecular Medicine, University of Oulu
P.O.Box 3000, FIN-90014 University of Oulu, Finland
University of Oulu, Aapistie 7A, 90220 Oulu, Finland
Tel. +358-(0)294 481199,
e-mail: rik.wierenga (at) oulu.fi
Focus area spearhead project web page
About the project
Our studies concern the characterisation of enzymes at the molecular level, including enzyme-substrate interactions. We study properties of CoA-dependent, thioester dependent lipid metabolising enzymes, such as acyl-CoA dehydrogenases, enoyl-CoA isomerases (ECIs), thiolases, multifunctional enzymes (MFE1, TFE), as well as enzymes that hydroxylate prolines of procollagen (collagen prolyl 4-hydroxylases, C-P4Hs). For these studies protein crystallographic approaches are complemented by biophysical characterisations, enzyme kinetics, computing and chemistry. The mechanistic studies concern better understanding of the chemistry of biocatalysis but also of the mechanisms of substrate channelling (MFE1, TFE) and processivity (C-P4H). These studies provide valuable expertise for future biotech and pharmaceutical applications.
Last updated: 15.10.2018