Leica SP8 FALCON laser scanning confocal microscope

Sensitive multi-position 3D time-lapse imaging of tissues, organotypic cultures and cells. Freely selectable excitation and emission wavelengths. Equipped with a resonant scanner - suitable for imaging rapidly occurring cellular events and for fast large area imaging. Real time adaptive deconvolution (LIGHTNING) to maximize resolution and contrast in captured images. Integrated Fluorescence Lifetime Imaging (FLIM).

Excitation lasers:
405nm diode, White Light Laser; supercontinuum 470nm-670nm
Detection:
Spectral fluorescence detectors (2 HyD, 1 PMT), motorized transmitted light detector (with DIC), confocal reflection imaging, Fast Fluorescence Lifetime Contrast (FALCON)
Objectives:
HC PL APO 10x/0.40 CS2 (Air),
HC PL APO 20x/0.75 CS2 (Air)
HC PL APO 20x/0.75 IMM Corr CS2 (W, Gly, Oil)
HC PL APO 40x/1.10 W motCORR CS2 (Water)
HC PL APO 63x/1.40 OIL CS2 DIC (Oil)
Scanner:
High resolution galvanometric and high speed resonant scanners
Incubator:
Okolab Cage Incubator for live cell/tissue time lapse imaging with temperature, humidity and CO2 control
Autofocus:
software or hardware-based
Software:
LAS X with LIGHTNING, Navigator and FLIM

A fully motorized inverted microscope and a stage for multi-position and tile-scan imaging. Suitable sample formats: slides, 35-60mm dishes, multi-well plates (6 to 96-well).

Last updated: 29.3.2019