Zeiss Cell Observer spinning disc confocal microscope

For live-cell imaging, molecular dynamics (FRAP) and cell-surface events detection (TIRF).

405nm, 488nm and 561nm lasers, metal-halide lamp
Hamamatsu EMCCD and CCD cameras, fluorescence filters:
Spinning disc: DAPI (BP 450/50nm), GFP (BP 525/50nm) and mCherry (BP 629/62nm)
TIRF: Filter Set 74 HE (DBP 525/31nm + 616/57nm) and Filter Set 76 HE (TBP 459nm + 525nm + 608nm)
EPI: DAPI (BP ex. 359/24nm, em. 445/50nm), GFP (BP ex. 470/20nm, em. 525/50nm), Texas Red (ex. 560/20nm, em. 630/75nm)
Transmitted light detection (DIC 25x-100x objectives/Phase contrast 10x objective)
Spinning disc unit:
Yokogawa CSU-X1
Plan NeoFluar 10x/0.30 Ph1 (air)
LD LCI Plan-Apochromat 25x/0.8 Imm. Korr DIC (water, oil, glycerine)
EC Plan NeoFluar 40x/0.75 DIC (air)
alpha Plan-Apochromat 63x/1.46 Korr DIC (oil)
alpha Plan-Apochromat 100x/1.46 DIC (oil)
objectives from Zeiss LSM 780 on request
software based or hardware-based DefiniteFocus
Top stage incubator for live cell/tissue time lapse imaging with temperature, humidity and CO2 control
Zen 2012 (Blue edition)

Total internal reflection fluorescence (TIRF) microscopy with 405nm, 488nm and 561nm excitation

Fluorescence recovery after photobleaching (FRAP), DirectFRAP-module

Fully motorized inverted microscope and stage for multi-position imaging

Suitable sample formats: slides, slide chambers, 35mm and 50mm dishes, 6-well and 24-well plates

Last updated: 28.4.2021