For live-cell imaging, molecular dynamics (FRAP) and cell-surface events detection (TIRF).
|
Excitation:
|
405nm, 488nm and 561nm lasers, metal-halide lamp
|
|
Detection:
|
Hamamatsu EMCCD and CCD cameras, fluorescence filters:
Spinning disc: DAPI (BP 450/50nm), GFP (BP 525/50nm) and mCherry (BP 629/62nm)
TIRF: Filter Set 74 HE (DBP 525/31nm + 616/57nm) and Filter Set 76 HE (TBP 459nm + 525nm + 608nm)
EPI: DAPI (BP ex. 359/24nm, em. 445/50nm), GFP (BP ex. 470/20nm, em. 525/50nm), Texas Red (ex. 560/20nm, em. 630/75nm)
Transmitted light detection (DIC 25x-100x objectives/Phase contrast 10x objective)
|
|
Spinning disc unit:
|
Yokogawa CSU-X1
|
|
Objectives:
|
Plan NeoFluar 10x/0.30 Ph1 (air)
LD LCI Plan-Apochromat 25x/0.8 Imm. Korr DIC (water, oil, glycerine) EC Plan NeoFluar 40x/0.75 DIC (air) alpha Plan-Apochromat 63x/1.46 Korr DIC (oil) alpha Plan-Apochromat 100x/1.46 DIC (oil) objectives from Zeiss LSM 780 on request |
|
Autofocus:
|
software based or hardware-based DefiniteFocus
|
|
Incubator:
|
Top stage incubator for live cell/tissue time lapse imaging with temperature, humidity and CO2 control
|
|
Software:
|
Zen 2012 (Blue edition)
|
Total internal reflection fluorescence (TIRF) microscopy with 405nm, 488nm and 561nm excitation
Fluorescence recovery after photobleaching (FRAP), DirectFRAP-module
Fully motorized inverted microscope and stage for multi-position imaging
Suitable sample formats: slides, slide chambers, 35mm and 50mm dishes, 6-well and 24-well plates
Last updated: 28.4.2021