Mass analysis is an indispensable step in the profound analysis of biomolecules, since nearly all modifications that happen to biomolecules are reflected in a change of their molecular weight, which can be measured with high sensitivity and accuracy with different mass spectrometers. In addition to “simple” molecular weight measurements, molecules can be fragmented within the mass spectrometer to provide structural information that allows identification and detailed characterization. Aim of the Biocenter Oulu core facility is to develop applications for mass spectrometry in biomedical research and to provide mass spectrometry service. Next to the routine mass spectrometry service, the facility is focused on identification and in depth characterization of proteins and their PTMs. The spectrum of potential samples covers purified proteins, complex proteomic samples, and proteins from 2D gel-based proteomics. General services include protein identification from protein mass finger prints or MSMS-spectra (sequencing), characterization of PTMS, accurate mass measurements of full length proteins, top down sequencing of proteins. Under development are methods to offer routine service for label free quantification of proteomes and targeted proteins.
The core facility is situated on the Kontinkangas campus in the Faculty of Biochemistry and Medical Biochemistry (F-block, Aapistie 7 room F-041A).
The major instruments of the core facility are a Thermo Orbitrap Fusion Lumos Tribrid, a Waters Synapt G2 HDMS Q-Tof and a Bruker UltrafleXtreme MALDI Tof/Tof. These cutting edge instruments can cover most applications in biological mass spectrometry.
Dr. Ulrich Bergmann
Faculty of Biochemistry and Molecular Medicine
University of Oulu
PL 3000, 90014 University of Oulu, Finland
tel. +358-(0)294 481209
e-mail: Ulrich.Bergmann (at) oulu.fi
Last updated: 5.2.2020