Protein crystallography

Biocenter Oulu offers services (contact persons) related to its expertise in protein crystallographic structure determination and provide support for researchers not familiar with structural studies to get the structure determination process started.

Protein sample

Characterization

Crystallization

Crystal quality check

Data collection

Structure determination

Structure analysis

Structure deposition

Our services and prices:

  • Characterization of proteins
     o Determination of molecular size with SLS instrument
     o Optimization of buffer content with DLS instrument
     o Study of protein stability with DSF instrument
  • Enzymology and ligand screening
     o Guiding and consultation of instrument usage

Events:

Contact persons:

Responsible scientists for the core facility:

Dr Lari Lehtiö
tel. +358-(0)294 481169 (office F126A)
e-mail: lari.lehtio (at) oulu.fi

Professor emeritus Rik Wierenga
tel. +358-(0)294 481199 (office F202B)
e-mail: rik.wierenga (at) oulu.fi

Postdoctoral scientist Tiila Kiema (data collection)
tel. +358-(0)294 481174 (office F217)
e-mail: tiila.kiema (at) oulu.fi

Postdoctoral scientist Kristian Koski
(remote data collection, courses, DLS, SLS, protein purification)
tel. +358-(0)294 481182 (office F216A)
e-mail: kristian.koski (at) oulu.fi

Project manager Outi Lampela
tel. +358-(0)294 486118 (office F005)
e-mail: outi.lampela (at) oulu.fi

Technical staff:

Ville Ratas (crystallization)
tel. +358-(0)294 481174 (office F216A)
e-mail: ville.ratas (at) oulu.fi

Ed Daniel (computing, IceBear)
tel. +358-(0)294 481151 (office F126B)
e-mail: edward.daniel (at) oulu.fi

Acknowledgement:

The Oulu BCO Instruct-FI infrastructure is supported by funding from Biocenter Finland, Biocenter Oulu, the University of Oulu, FBMM, and regional funding. We kindly ask the users to acknowledge this resource whenever publications are being finalised, for example:

"The use of the facilities and expertise of the Biocenter Oulu Protein Crystallography core facility, a member of Biocenter Finland and Instruct-FI, is gratefully acknowledged."

In addition we ask the users to inform us (i) whenever such publications have been accepted for publication and (ii) of the PDB entry codes whenever structures have been deposited.

 

Last updated: 22.1.2019