Storage of mouse lines by cryopreservation

Cryopreservation of sperm

Archiving includes cryopreservation and storage of sperm in liquid nitrogen.

The customer is responsible for

  • providing 2-3 males
  • the genotyping of embryos or pups, in case of quality control by IVF

Service includes the cryopreservation of sperm, and the quality control of the frozen sperm by IVF, followed by either in vitro culture or transfer of 2-cell embryos generated.

Cryopreservation of embryos

Cryopreserved embryos are generated either by IVF or by mating.

The customer is responsible for:

  • providing 2-3 males (in case of IVF)
  • providing both 2-3 males and 15 females (3-5 week-old) carrying the correct genotype (in case of mating)
  • the genotyping of embryos or pups  

Service includes cryopreservation of both sperm and 2-cell embryos (up to 200) generated by IVF and as quality control either in vitro culture or transfer of 2-cell embryos.

Service includes cryopreservation of embryos generated by mating. The project includes the superovulation of 15 oocyte donors, the isolation and cryopreservation of the embryos and as quality control either in vitro culture or transfer of embryos.

Last updated: 30.3.2020